Rat Dopamine Receptor D4 (DRD4) ELISA Kit | DRD4 elisa kit

Rat Dopamine Receptor D4 (DRD4) ELISA Kit

Cat. Num. AAA2023640

• Gene Names: DRD4; D4DR
• Reactivity: Rat
• Synonyms: Dopamine Receptor D4 (DRD4); Rat Dopamine Receptor D4 (DRD4) ELISA Kit; DR-D4; D4R; D4DR; D(2C) dopamine receptor; DRD4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dopamine Receptor D4 (DRD4).; No significant cross-reactivity or interference between Dopamine Receptor D4 (DRD4) and analogues was observed.
• Sequence Length: 467

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine Receptor D4 (DRD4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine Receptor D4 (DRD4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DRD4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Receptor D4 (DRD4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Receptor D4 (DRD4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Receptor D4 (DRD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopamine Receptor D4 (DRD4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DRD4 elisa kit

Signal transduction; Infection immunity; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 291946
• NCBI GeneID: 1815
• UniProt Accession #: P21917; Q7Z7Q5; Q8NGM5; B0M0J7
• Molecular Weight: 48,361 Da
• NCBI Official Full Name: dopamine receptor D4
• NCBI Official Synonym Full Names: dopamine receptor D4
• NCBI Official Symbol: DRD4
• NCBI Official Synonym Symbols: D4DR
• NCBI Protein Information: D(4) dopamine receptor
• UniProt Protein Name: D(4) dopamine receptor
• Protein Family: D(4) dopamine receptor
• UniProt Gene Name: DRD4
• UniProt Entry Name: DRD4_HUMAN

NCBI Description

This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats. [provided by RefSeq, Jul 2008]

Uniprot Description

DRD4: Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Its activity is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: membrane; cell soma; integral to plasma membrane; dendritic spine; plasma membrane; terminal button; cell cortex; vesicle membrane

Molecular Function: dopamine D2 receptor-like receptor activity; identical protein binding; protein binding; potassium channel regulator activity; dopamine receptor activity; dopamine binding; drug binding; SH3 domain binding

Biological Process: circadian rhythm; fear response; synaptic transmission, dopaminergic; behavioral fear response; positive regulation of dopamine uptake; adult locomotory behavior; activation of MAPK activity; short-term memory; positive regulation of kinase activity; regulation of neurotransmitter secretion; regulation of calcium-mediated signaling; behavioral response to ethanol; dopamine metabolic process; dopamine receptor, adenylate cyclase inhibiting pathway; regulation of inhibitory postsynaptic membrane potential; negative regulation of cAMP biosynthetic process; behavioral response to cocaine; negative regulation of protein secretion; regulation of dopamine metabolic process; response to amphetamine; negative regulation of adenylate cyclase activity; social behavior; regulation of circadian rhythm; arachidonic acid secretion; cellular calcium ion homeostasis; olfactory learning; response to steroid hormone stimulus; retina development in camera-type eye; photoperiodism; dopamine receptor signaling pathway; positive regulation of sodium:hydrogen antiporter activity

Disease: Novelty Seeking Personality Trait; Attention Deficit-hyperactivity Disorder