Human Protein Tyrosine Phosphatase Receptor Type S (PTPRS) ELISA Kit | PTPRS elisa kit

Human Protein Tyrosine Phosphatase Receptor Type S (PTPRS) ELISA Kit

Cat. Num. AAA2703138

• Gene Names: PTPRS; R-PTP-S; PTPSIGMA; R-PTP-sigma
• Reactivity: Human
• Synonyms: Protein Tyrosine Phosphatase Receptor Type S (PTPRS); Human Protein Tyrosine Phosphatase Receptor Type S (PTPRS) ELISA Kit; PTPR-S; PTPSIGMA; PTP SIGMA; Receptor-type tyrosine-protein phosphatase sigma; PTPRS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Tyrosine Phosphatase Receptor Type S (PTPRS).; No significant cross-reactivity or interference between Protein Tyrosine Phosphatase Receptor Type S (PTPRS) and analogues was observed.
• Sequence Length: 1,501

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: < 0.27ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Tyrosine Phosphatase Receptor Type S (PTPRS) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Tyrosine Phosphatase Receptor Type S (PTPRS) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTPRS elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase Receptor Type S (PTPRS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase Receptor Type S (PTPRS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Phosphatase Receptor Type S (PTPRS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Tyrosine Phosphatase Receptor Type S (PTPRS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119589584
• NCBI GeneID: 5802
• UniProt Accession #: Q13332; O75255; O75870; Q15718; Q16341; Q2M3R7
• Molecular Weight: 168,394 Da
• NCBI Official Full Name: protein tyrosine phosphatase, receptor type, S, isoform CRA_h
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type S
• NCBI Official Symbol: PTPRS
• NCBI Official Synonym Symbols: R-PTP-S; PTPSIGMA; R-PTP-sigma
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase S
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase S
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: PTPRS
• UniProt Synonym Gene Names: R-PTP-S; R-PTP-sigma

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of multiple Ig-like and fibronectin type III-like domains. Studies of the similar gene in mice suggested that this PTP may be involved in cell-cell interaction, primary axonogenesis, and axon guidance during embryogenesis. This PTP has been also implicated in the molecular control of adult nerve repair. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Cell surface receptor that binds to glycosaminoglycans, including chondroitin sulfate proteoglycans and heparan sulfate proteoglycan (PubMed:21454754). Binding to chondroitin sulfate and heparan sulfate proteoglycans has opposite effects on PTPRS oligomerization and regulation of neurite outgrowth. Contributes to the inhibition of neurite and axonal outgrowth by chondroitin sulfate proteoglycans, also after nerve transection. Plays a role in stimulating neurite outgrowth in response to the heparan sulfate proteoglycan GPC2. Required for normal brain development, especially for normal development of the pituitary gland and the olfactory bulb. Functions as tyrosine phosphatase (PubMed:8524829). Mediates dephosphorylation of NTRK1, NTRK2 and NTRK3 (). Plays a role in down-regulation of signaling cascades that lead to the activation of Akt and MAP kinases (). Down-regulates TLR9-mediated activation of NF-kappa-B, as well as production of TNF, interferon alpha and interferon beta (PubMed:26231120).