Mouse Thymidine Phosphorylase Monoclonal Antibody | anti-Tymp antibody

Thymidine Phosphorylase (TYMP, ECGF1, Gliostatin, hPD-ECGF, MNGIE, PDECGF, PD-ECGF, Platelet-derived endothelial cell growth factor, TdRPase, TP)

Cat. Num. AAA601425

• Gene Names: Tymp; Ecgf1; PDECGF; Pdgfec; PD-ECGF; 2900072D10Rik
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Neutralization Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Thymidine Phosphorylase; Monoclonal Antibody; Thymidine Phosphorylase (TYMP; ECGF1; Gliostatin; hPD-ECGF; MNGIE; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; TP); Anti -Thymidine Phosphorylase (TYMP; anti-Tymp antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 0.N.568
• Specificity: Recognizes human, mouse and rat thymidine phosphorylase.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-Tymp antibody: Immunohistochemistry (IHC), Neutralization
• Application Notes: Suitable for use in Immunohistochemistry (formalin/ paraffin)., Note: As reported by an independent laboratory, neutralizes enzymatic activity of TP/PD-ECGF.; Dilution: Immunohistochemistry: 1-2ug/ml for 30 minutes at RT. No special treatment is required for immunohistochemical staining of formalin fixed tissues.
• Immunogen: Recombinant full length human thymidine phosphorylase (TP/PD-ECGF) protein.
• Cellular Localization: Cytoplasmic and nuclear
• Positive Control: HUVEC cells or breast carcinoma
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained using MBS601425, followed by peroxidase-conjugate and AEC chromogen. Note nuclear and cytoplasmic staining of tumor cells.)

Related Product Information for anti-Tymp antibody

In the presence of inorganic orthophosphate, thymidine phosphorylase catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5'-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Product Categories/Family for anti-Tymp antibody

Antibodies; Abs to Enzymes; Phosphorylase

NCBI and Uniprot Product Information

• NCBI GI #: 19923857
• NCBI GeneID: 72962
• NCBI Accession #: NP_612175.1
• NCBI GenBank Nucleotide #: NM_138302.1
• UniProt Accession #: Q99N42
• Molecular Weight: 55kD (reduced)
• NCBI Official Full Name: thymidine phosphorylase
• NCBI Official Synonym Full Names: thymidine phosphorylase
• NCBI Official Symbol: Tymp
• NCBI Official Synonym Symbols: Ecgf1; PDECGF; Pdgfec; PD-ECGF; 2900072D10Rik
• NCBI Protein Information: thymidine phosphorylase; TP; tdRPase; gliostatin; platelet derived growth factor, endothelial cell; endothelial cell growth factor 1 (platelet-derived)
• UniProt Protein Name: Thymidine phosphorylase
• Protein Family: Thymidine phosphorylase
• UniProt Gene Name: Tymp
• UniProt Synonym Gene Names: Ecgf1
• UniProt Entry Name: TYPH_MOUSE

Uniprot Description

ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Protein type: Nucleotide Metabolism - pyrimidine; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; Phosphorylase; Motility/polarity/chemotaxis; Cytokine; EC 2.4.2.4; DNA replication

Molecular Function: transferase activity; transferase activity, transferring glycosyl groups; thymidine phosphorylase activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity

Biological Process: organ morphogenesis; pyrimidine base metabolic process; metabolic process; chemotaxis; pyrimidine nucleoside metabolic process