Western Blot (WB)
(Western blot analysis of extracts from COS cells, untransfected or transfected with human ECGF1, and human THP-1 cells, using MBS606212.)
Rabbit anti-Human Thymidine Phosphorylase Monoclonal Antibody | anti-Tymp antibody
Thymidine Phosphorylase (TYMP, ECGF1, Gliostatin, hPD-ECGF, MNGIE, PDECGF, PD-ECGF, Platelet-derived endothelial cell growth factor, TdRPase, TP)
Gene Names
Tymp; Ecgf1; PDECGF; Pdgfec; PD-ECGF; 2900072D10Rik
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Purified
Synonyms
Thymidine Phosphorylase; Monoclonal Antibody; Thymidine Phosphorylase (TYMP; ECGF1; Gliostatin; hPD-ECGF; MNGIE; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; TP); Anti -Thymidine Phosphorylase (TYMP; anti-Tymp antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
D69B12
Specificity
Recongnizes endogenous levels of human total TP/ECGF1 protein.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-Tymp antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Optimal dilutions should be determined by the researcher.
Other applications not tested.
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Optimal dilutions should be determined by the researcher.
Other applications not tested.
Immunogen
Synthetic peptide corresponding to residues surrounding Gln370 of human thymidine phosphorylase/ECGF1 protein (KLH-coupled).
Preparation and Storage
Store at -20°C. Aliquots are stable for 12 months after receipt.
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of extracts from COS cells, untransfected or transfected with human ECGF1, and human THP-1 cells, using MBS606212.)
Western Blot (WB)
(Western blot analysis of extracts from COS cells, untransfected or transfected with human ECGF1, and human THP-1 cells, using MBS606212.)
Related Product Information for anti-Tymp antibody
Thymidine phosphorylase (TP) is a platelet-derived endothelial cell growth factor (PD-ECGF) that catalyzes the formation of thymine and 2-deoxy-D-ribose-1-phosphate from thymidine and orthophosphate. This intracellular enzyme is capable of both promoting angio-genesis and inhibiting apoptosis. Thymidine phosphorylase catalytic activity is required for its angiogenic function. Increased expression of TP/PD-ECGF is seen in a wide vari-ety of different solid tumors and inflammatory diseases and is often associated with poor prognosis. Alternatively, TP can activate fluorouracil derivative (DFUR) prodrugs and increase the antitumor activity of the related treatment. The use of thymidine phosphorylase as a cancer therapeutic target has been studied extensively, with emphasis on either inhibiting TP enzymatic activity or increasing enzyme induc-tion with concomitant DFUR treatment.
Product Categories/Family for anti-Tymp antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thymidine phosphorylase
NCBI Official Synonym Full Names
thymidine phosphorylase
NCBI Official Symbol
Tymp
NCBI Official Synonym Symbols
Ecgf1; PDECGF; Pdgfec; PD-ECGF; 2900072D10Rik
NCBI Protein Information
thymidine phosphorylase; TP; tdRPase; gliostatin; platelet derived growth factor, endothelial cell; endothelial cell growth factor 1 (platelet-derived)
UniProt Protein Name
Thymidine phosphorylase
Protein Family
UniProt Gene Name
Tymp
UniProt Synonym Gene Names
Ecgf1
UniProt Entry Name
TYPH_MOUSE
Uniprot Description
ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
Protein type: Phosphorylase; Motility/polarity/chemotaxis; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; EC 2.4.2.4; DNA replication; Nucleotide Metabolism - pyrimidine; Cytokine
Molecular Function: transferase activity; transferase activity, transferring glycosyl groups; thymidine phosphorylase activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity
Biological Process: organ morphogenesis; pyrimidine base metabolic process; metabolic process; chemotaxis; pyrimidine nucleoside metabolic process
Research Articles on Tymp
Similar Products
Product Notes
The Tymp tymp (Catalog #AAA606212) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Thymidine Phosphorylase (TYMP, ECGF1, Gliostatin, hPD-ECGF, MNGIE, PDECGF, PD-ECGF, Platelet-derived endothelial cell growth factor, TdRPase, TP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Thymidine Phosphorylase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions should be determined by the researcher. Other applications not tested. Researchers should empirically determine the suitability of the Tymp tymp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thymidine Phosphorylase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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