Western Blot (WB)
(Western blot analysis of TCTP on different lysates using anti-TCTP antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse placenta tissue Lane 2: Mouse testis tissue Lane 3: Hela Lane 4: A431 Lane 5: MCF-7)
Rabbit TCTP Monoclonal Antibody | anti-TCTP antibody
TCTP Antibody
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50-1:100
Western Blot (WB)
(Western blot analysis of TCTP on different lysates using anti-TCTP antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse placenta tissue Lane 2: Mouse testis tissue Lane 3: Hela Lane 4: A431 Lane 5: MCF-7)
Western Blot (WB)
(Western blot analysis of TCTP on different lysates using anti-TCTP antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse placenta tissue Lane 2: Mouse testis tissue Lane 3: Hela Lane 4: A431 Lane 5: MCF-7)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TCTP antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TCTP antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TCTP antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TCTP antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining TCTP in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining TCTP in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining TCTP in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining TCTP in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining TCTP in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining TCTP in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of THP-1 cells with TCTP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of THP-1 cells with TCTP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
Uniprot Description
TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation.
Protein type: Microtubule-binding; Calcium-binding
Chromosomal Location of Human Ortholog: 13q14
Cellular Component: multivesicular body; extracellular space; tubulin complex; cytoplasm; nucleus
Molecular Function: protein binding; calcium ion binding
Biological Process: cellular calcium ion homeostasis; regulation of apoptosis; response to virus; calcium ion transport; negative regulation of apoptosis
Research Articles on TCTP
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Product Notes
The TCTP tpt1 (Catalog #AAA9436474) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TCTP Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TCTP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the TCTP tpt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCTP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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