Mouse anti-Human, Mouse TP73 / p73 Monoclonal Antibody | anti-TP73 antibody

Mouse Monoclonal [clone 5B429] (IgG) to Human TP73 / p73

Cat. Num. AAA245193

• Gene Names: TP73; P73
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation
• Purity: Protein G Purified
• Synonyms: TP73 / p73; Monoclonal Antibody; Mouse Monoclonal [clone 5B429] (IgG) to Human TP73 / p73; Anti-TP73 / p73 Antibody (N-Terminus; clone 5B429) IHC-plus; TP73; p53-like transcription factor; p73; p53-related protein; Tumor protein p73; Human TP73; anti-TP73 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 5B429
• Specificity: Full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
• Purity/Purification: Protein G Purified
• Form/Format: PBS containing 0.05% BSA and 0.05% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 636

• Applicable Applications for anti-TP73 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: ICC, IHC-P (5 ug/ml), IP, WB (2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
• Immunogen: TP73 / p73 antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-p73 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of p73 in A) transfected?cell lysate and B) HeLa cell lysate using antibody at 2 ug/ml.)

Related Product Information for anti-TP73 antibody

This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 4885645
• NCBI GeneID: 7161
• NCBI Accession #: NP_005418.1
• NCBI GenBank Nucleotide #: NM_005427.3
• UniProt Accession #: O15350; O15351; Q17RN8; Q5TBV5; Q5TBV6; Q8NHW9; Q8TDY5; Q8TDY6; B7Z7J4; B7Z8Z1; B7Z9C1; C9J521
• Molecular Weight: 61,694 Da
• NCBI Official Full Name: tumor protein p73 isoform a
• NCBI Official Synonym Full Names: tumor protein p73
• NCBI Official Symbol: TP73
• NCBI Official Synonym Symbols: P73
• NCBI Protein Information: tumor protein p73; p53-related protein; p53-like transcription factor
• UniProt Protein Name: Tumor protein p73
• Protein Family: Tumor protein
• UniProt Gene Name: TP73
• UniProt Synonym Gene Names: P73
• UniProt Entry Name: P73_HUMAN

NCBI Description

This gene encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined. [provided by RefSeq, Feb 2011]

Uniprot Description

p73: a protein of the p53 family of proteins. p73, like p53, may have tumor suppressor activity, but its multiple isoforms possess different and sometimes opposing functions. Participates in the apoptotic response to DNA damage. Phosphorylated in a cell cycle-dependent manner and negatively regulated by CDKs. When overproduced, activates transcription from p53-responsive promoters and induces apoptosis. Nine transactivation competent (TA) or dominant negative (DN) isoforms, derived from alternative-splicing and alternative promoters, have been described. NH2-terminally truncated p73 isoforms (DNp73) may be oncogenic. DNp73 is elevated in a number of cancers and is associated with adverse clinical outcomes and resistance to chemotherapy. The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9; forms complex with p53 and CABLES1. Isoform Beta interacts with WWOX; interacts with HECW2.

Protein type: DNA-binding; Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: nucleoplasm; Golgi apparatus; transcription factor complex; intracellular membrane-bound organelle; cell junction; chromatin; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; p53 binding; metal ion binding; double-stranded DNA binding; damaged DNA binding; chromatin binding; transcription factor activity; transcription factor binding; protein kinase binding

Biological Process: G1 DNA damage checkpoint; transcription from RNA polymerase II promoter; cerebrospinal fluid secretion; positive regulation of cell size; activation of MAPK activity; positive regulation of transcription, DNA-dependent; regulation of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; post-embryonic development; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; negative regulation of cardiac muscle cell proliferation; response to gamma radiation; negative regulation of neuron apoptosis; positive regulation of oligodendrocyte differentiation; cell cycle arrest; kidney development; inflammatory response; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; protein tetramerization; response to X-ray; negative regulation of JNK activity; release of cytochrome c from mitochondria; mismatch repair; hippocampus development; digestive tract morphogenesis; response to organic nitrogen; negative regulation of neuron differentiation; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of gene expression; neuron development; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus