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Mouse anti-Rat TCR gamma/delta Monoclonal Antibody | anti-TCRgamma/delta antibody

Anti-Rt TCR gamma/delta Purified Low Endotoxin

Reactivity
Rat
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry
Purity
Purified by protein-A affinity chromatography
Synonyms
TCR gamma/delta; Monoclonal Antibody; Anti-Rt TCR gamma/delta Purified Low Endotoxin; TCRG/D; anti-TCRgamma/delta antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
V65
Specificity
The mouse monoclonal antibody V65 recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
Purity/Purification
Purified by protein-A affinity chromatography
Form/Format
Purified Low Endotoxin. Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TCRgamma/delta antibody
Functional Application (FUNC), Flow Cytometry (FC/FACS) (QC tested), Immunoprecipitation (IP), Immunohistochemistry-Frozen (IHC-F)
Application Notes
FC/FACS: Recommended dilution: 1-4ug/ml
Immunogen
TCR alpha/beta-negative CD3-positive rat T cell hybridoma III.89.1.4 line
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.
Related Product Information for anti-TCRgamma/delta antibody
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form Functional Application (FUNC)tional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
References
*De Bernardis F, Lucciarini R, Boccanera M, Amantini C, Arancia S, Morrone S, Mosca M, Cassone A, Santoni G: Phenotypic and Functional Application (FUNC)tional characterization of vaginal dendritic cells in a rat model of Candida albicans vaginitis. Infect Immun. 2006 Jul;74(7):4282-94., URL: http://www.ncbi.nlm.nih.gov/pubmed/16790803,*van den Brandt J, Kwon SH, Hünig T, McPherson KG, Reichardt HM: Sustained pre-TCR expression in Notch1IC-transgenic rats impairs T cell maturation and selection. J Immunol. 2005 Jun 15;174(12):7845-52., URL: http://www.ncbi.nlm.nih.gov/pubmed/15944289,*Moghaddami M, Cleland LG, Mayrhofer G: MHC II+ CD45+ cells from synovium-rich tissues of normal rats: phenotype, comparison with macrophage and dendritic cell lineages and differentiation into mature dendritic cells in vitro. Int Immunol. 2005 Aug;17(8):1103-15., URL: https://www.ncbi.nlm.nih.gov/pubmed/16030130,*Xystrakis E, Dejean AS, Bernard I, Druet P, Liblau R, Gonzalez-Dunia D, Saoudi A: Identification of a novel natural regulatory CD8 T-cell subset and analysis of its mechanism of regulation. Blood. 2004 Nov 15;104(10):3294-301., URL: https://www.ncbi.nlm.nih.gov/pubmed/15271801,*Henriksson G, Helgeland L, Midtvedt T, Stierna P, Brandtzaeg P: Immune response to Mycoplasma pulmonis in nasal mucosa is modulated by the normal microbiota. Am J Respir Cell Mol Biol. 2004 Dec;31(6):657-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/15347559,*Tam FW, Smith J, Morel D, Karkar AM, Thompson EM, Cook HT, Pusey CD: Development of scarring and renal failure in a rat model of crescentic glomerulonephritis. Nephrol Dial Transplant. 1999 Jul;14(7):1658-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/10435873,*Hosea HJ, Rector ES, Taylor CG: Zinc-deficient rats have fewer recent thymic emigrant (CD90+) T lymphocytes in spleen and blood. J Nutr. 2003 Dec;133(12):4239-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/14652378,*Giezeman-Smits KM, Jonges LE, Chambers WH, Brisette-Storkus CS, Van Vlierberghe RL, Van Eendenburg JD, Eggermont AM, Fleuren GJ, Kuppen PJ: Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL-2-activated rat NK cells. J Leukoc Biol. 1998 Feb;63(2):209-15., URL: https://www.ncbi.nlm.nih.gov/pubmed/9468279

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Product Notes

The TCRgamma/delta (Catalog #AAA1801848) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rt TCR gamma/delta Purified Low Endotoxin reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TCR gamma/delta can be used in a range of immunoassay formats including, but not limited to, Functional Application (FUNC), Flow Cytometry (FC/FACS) (QC tested), Immunoprecipitation (IP), Immunohistochemistry-Frozen (IHC-F). FC/FACS: Recommended dilution: 1-4ug/ml. Researchers should empirically determine the suitability of the TCRgamma/delta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR gamma/delta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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