anti-Mouse TCR gamma delta Monoclonal Antibody

Hamster Anti-Mouse TCRgd-BIOT

Cat. Num. AAA673499

• Reactivity: Mouse
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: TCR gamma delta; Monoclonal Antibody; Hamster Anti-Mouse TCRgd-BIOT; Hamster Anti-Mouse TCRgd - Biotin; Hamster Anti-Mouse TCR gamma delta; anti-TCR gamma delta antibody

• Host: Armenian Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Armenian Hamster IgG
• Clone Number: UC7-13D5
• Specificity: gamma delta T-cell receptor (TCR) complex; The TCR gamma delta heterodimer of the CD3/T-cell receptor complex is clonotypic and consists of Ig-like variable and constant domains.1 It is expressed during thymopoiesis and on the small subpopulation of gamma delta TCRexpressing T lymphocytes in the periphery. The majority of T cells present in some epithelial tissues are gamma delta + and have limited receptor diversity.1, 2 The TCR gamma delta heterodimer recognizes peptide antigen bound to MHC antigens, and subsequent signal transduction mediated by the invariant chains leads to T cell activation.3 The monoclonal antibody UC7-13D5 is specific for the gamma delta heterodimer and plate-bound UC7-13D5 activates gamma delta TCR-bearing cells.4-7 This antibody does not react with alpha beta TCR-expressing T cells.
• Form/Format: Biotin (BIOT) Conjugate

• Applicable Applications for anti-TCR gamma delta antibody: Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
• Application Notes: Research Application(s): In vitro activation of gamma delta TCR-bearing cells; In vivo depletion of gamma delta TCR-expressing T cells; ; Flow Cytometry Suggested Dilution: <= 0.3 ug/10^6 cells
• Characterization: To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Davis, M.M. 1990. Annu. Rev. Biochem. 59:475.
2. Allison, J.P., and W.L. Havran. 1991. Annu. Rev. Immunol. 9:679.
3. Klausner, R.D., and L.E. Samuelson. 1991. Cell 64:875.
4. Dent, A.L., et al. 1990. Nature 343:714.
5. Bluestone, J.A., et al. 1991. Immunol. Rev. 120:5.
6. Houlden, B.A., et al. 1989. Cold Spring Harbor Symp. Quant. Biol. 54:45.
7. Barrett, T.A., et al. 1991. Immunol. Res. 10:341.
8. Unpublished observations.