Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Immunohistochemistry (IHC) (Anti-TCL / RHOJ antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Mouse anti-Human TCL / RHOJ Monoclonal Antibody | anti-TCL / RHOJ antibody

Anti-TCL / RHOJ Antibody (clone 1D7) IHC-plus

Gene Names
RHOJ; TCL; ARHJ; TC10B; RASL7B
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Protein A/G purified
Synonyms
TCL / RHOJ; Monoclonal Antibody; Anti-TCL / RHOJ Antibody (clone 1D7) IHC-plus; Mouse Monoclonal [clone 1D7] (IgG2a) to Human TCL / RHOJ; Human TCL / RHOJ; ARHJ; Ras homolog family member J; TC10-like Rho GTPase; TC10B; RAS-like; family 7; member B; RASL7B; RHOI; Tc10-like GTP-binding protein; anti-TCL / RHOJ antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1D7
Specificity
Human TCL / RHOJ
Purity/Purification
Protein A/G purified
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
214
Applicable Applications for anti-TCL / RHOJ antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF (1:100)
IHC-P (10 ug/ml)
WB (1:2000)
Immunogen
TCL / RHOJ antibody was raised against full length human recombinant protein of human RHOJ(NP_065714) produced in HEK293T cell.
Immunogen Description
Full length human recombinant protein of human RHOJ(NP_065714) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Target Species
Human
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TCL / RHOJ antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC) (Anti-TCL / RHOJ antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Anti-RHOJ mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RHOJ.)

Immunofluorescence (IF) (Anti-RHOJ mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RHOJ.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RHOJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RHOJ.)

Western Blot (WB) (HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RHOJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RHOJ.)
Related Product Information for anti-TCL / RHOJ antibody
RHOJ Antibody, ARHJ Antibody, Ras homolog family member J Antibody, TC10-like Rho GTPase Antibody, TC10B Antibody, RAS-like, family 7, member B Antibody, RASL7B Antibody, RHOI Antibody, Tc10-like GTP-binding protein Antibody, TCL Antibody Description: GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,255 Da
NCBI Official Full Name
rho-related GTP-binding protein RhoJ
NCBI Official Synonym Full Names
ras homolog family member J
NCBI Official Symbol
RHOJ
NCBI Official Synonym Symbols
TCL; ARHJ; TC10B; RASL7B
NCBI Protein Information
rho-related GTP-binding protein RhoJ
UniProt Protein Name
Rho-related GTP-binding protein RhoJ
UniProt Gene Name
RHOJ
UniProt Synonym Gene Names
ARHJ; RASL7B; RHOI; TCL
UniProt Entry Name
RHOJ_HUMAN

NCBI Description

This gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011]

Uniprot Description

RHOJ: GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology. Belongs to the small GTPase superfamily. Rho family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric; Motility/polarity/chemotaxis; G protein; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: cytosol; plasma membrane

Molecular Function: GTP binding; GTPase activity; protein binding

Biological Process: actin cytoskeleton organization and biogenesis; regulation of cell shape; regulation of small GTPase mediated signal transduction; Rho protein signal transduction; small GTPase mediated signal transduction

Research Articles on TCL / RHOJ

Similar Products

Product Notes

The TCL / RHOJ rhoj (Catalog #AAA249281) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-TCL / RHOJ Antibody (clone 1D7) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCL / RHOJ can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB). IF (1:100) IHC-P (10 ug/ml) WB (1:2000). Researchers should empirically determine the suitability of the TCL / RHOJ rhoj for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCL / RHOJ, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.