Rabbit anti-Human RHOJ Polyclonal Antibody | anti-RHOJ antibody

RHOJ, ID (RHOJ, ARHJ, RASL7B, RHOI, TCL, Rho-related GTP-binding protein RhoJ, Ras-like protein family member 7B, Tc10-like GTP-binding protein) (PE)

Cat. Num. AAA6341993

• Gene Names: RHOJ; TCL; ARHJ; TC10B; RASL7B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RHOJ; Polyclonal Antibody; ID (RHOJ; ARHJ; RASL7B; RHOI; TCL; Rho-related GTP-binding protein RhoJ; Ras-like protein family member 7B; Tc10-like GTP-binding protein) (PE); Tc10-like GTP-binding protein; anti-RHOJ antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RHOJ antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RHOJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the Central region of human RHOJ.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(RHOJ Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RHOJ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(RHOJ Antibody (Center) western blot analysis in HepG2,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RHOJ antibody detected the RHOJ protein (arrow).)

Related Product Information for anti-RHOJ antibody

ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis.

Product Categories/Family for anti-RHOJ antibody

Antibodies; Binding Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Lamesch, P., et al. Genomics 89(3):307-315(2007). Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005). Nishikimi, A., et al. FEBS Lett. 579(5):1039-1046(2005). Ruusala, A., et al. FEBS Lett. 572 (1-3), 159-166 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 16903164
• NCBI GeneID: 57381
• NCBI Accession #: NP_065714.1
• NCBI GenBank Nucleotide #: NM_020663.4
• UniProt Accession #: Q9H4E5; Q96KC1
• Molecular Weight: 21,255 Da
• NCBI Official Full Name: rho-related GTP-binding protein RhoJ
• NCBI Official Synonym Full Names: ras homolog family member J
• NCBI Official Symbol: RHOJ
• NCBI Official Synonym Symbols: TCL; ARHJ; TC10B; RASL7B
• NCBI Protein Information: rho-related GTP-binding protein RhoJ
• UniProt Protein Name: Rho-related GTP-binding protein RhoJ
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RHOJ
• UniProt Synonym Gene Names: ARHJ; RASL7B; RHOI; TCL
• UniProt Entry Name: RHOJ_HUMAN

NCBI Description

This gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011]

Uniprot Description

RHOJ: GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology. Belongs to the small GTPase superfamily. Rho family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric; Motility/polarity/chemotaxis; G protein; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: cytosol; plasma membrane

Molecular Function: GTP binding; GTPase activity; protein binding

Biological Process: actin cytoskeleton organization and biogenesis; regulation of cell shape; regulation of small GTPase mediated signal transduction; Rho protein signal transduction; small GTPase mediated signal transduction