Immunofluorescence (IF)
( Immunofluorescence staining of fixed U251 cells with anti-tau antibody Tau-5 (Ab00639) Immunofluorescence analysis of paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Tau-5 (Ab0639-1.1) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00639-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (Ab178-1.1) followed by Alexa Fluor® 488 secondary antibody.)
tau recombinant antibody
Anti-tau [Tau-5]
Reactivity
Human, Mouse, Rat, Sheep
Applications
Immunofluorescence
Purity
Protein A Affinity Purified
Synonyms
tau; Monoclonal Recombinant Antibody; Anti-tau [Tau-5]; microtubule-associated protein tau; tau recombinant antibody
Reactivity
Human, Mouse, Rat, Sheep
Clonality
Monoclonal
Isotype
Mouse IgG1, Kappa
Clone Number
Tau-5
Specificity
This antibody binds to sequence 210-241 of tau protein.
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Applicable Applications for tau recombinant antibody
Immunofluorescence (IF), Electron Microscopy (EM)
Application Notes
This antibody binds to tau in a phosphorylation independent manner. Tau proteins are abundant in the central nervous system and stabilize micrtubules. Immuno-staining can be affected by different fixation methods.
Immunogen
Tau protein.
Buffer Composition
PBS only with 0.02% Proclin 300.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
( Immunofluorescence staining of fixed U251 cells with anti-tau antibody Tau-5 (Ab00639) Immunofluorescence analysis of paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Tau-5 (Ab0639-1.1) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00639-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (Ab178-1.1) followed by Alexa Fluor® 488 secondary antibody.)
Immunofluorescence (IF)
( Immunofluorescence staining of fixed U251 cells with anti-tau antibody Tau-5 (Ab00639) Immunofluorescence analysis of paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Tau-5 (Ab0639-1.1) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00639-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (Ab178-1.1) followed by Alexa Fluor® 488 secondary antibody.)
Related Product Information for tau recombinant antibody
Recombinant monoclonal antibody to tau. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Tau-5.
Tau protein.
Tau protein.
Product Categories/Family for tau recombinant antibody
References
LoPresti P, Szuchet S, Papasozomenos SC, Zinkowski RP, Binder LI. Functional implications for the microtubule-associated protein tau: localization in oligodendrocytes. Proc Natl Acad Sci U S A. 1995 Oct 24; 92(22):10369-73. PMID:7479786
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Product Notes
The tau (Catalog #AAA488335) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-tau [Tau-5] reacts with Human, Mouse, Rat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's tau can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Electron Microscopy (EM). This antibody binds to tau in a phosphorylation independent manner. Tau proteins are abundant in the central nervous system and stabilize micrtubules. Immuno-staining can be affected by different fixation methods. Researchers should empirically determine the suitability of the tau for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "tau, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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