Western Blot (WB)
(Figure 1: Western blot analysis using SYT1 mouse mAb against truncated Trx-SYT1 recombinant protein (1).)
Mouse anti-Human SYT1 Monoclonal Antibody | anti-SYT1 antibody
SYT1 Antibody
Gene Names
SYT1; P65; SYT; SVP65
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-Chromatography
Synonyms
SYT1; Monoclonal Antibody; SYT1 Antibody; DKFZp781D2042; FLJ42519; p65; SVP65; Synaptotagmin 2; Synaptotagmin I; Synaptotagmin II; Synaptotagmin-1; SYT; Syt1; SYT1_HUMAN; Syt2; SytI; Synaptotagmin-2; Synaptotagmin2; SynaptotagminII; SYT 2; Syt II; SYT2_HUMAN; SytII; anti-SYT1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
SYT1 antibody detects endogenous levels of total SYT1
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
422
Applicable Applications for anti-SYT1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
WB: 1:500-1:2000
Immunogen
Purified recombinant fragment of human SYT1 expressed in E. Coli
Subcellular Location
Cytoplasmic Vesicle > Secretory Vesicle > Synaptic Vesicle Membrane. Cytoplasmic Vesicle > Secretory Vesicle > Chromaffin Granule Membrane. Cytoplasm. Synaptic vesicles and chromaffin granules.
Tissue Specificity
Expressed in melanocytes (PubMed:23999003).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using SYT1 mouse mAb against truncated Trx-SYT1 recombinant protein (1).)
Western Blot (WB)
(Figure 1: Western blot analysis using SYT1 mouse mAb against truncated Trx-SYT1 recombinant protein (1).)
Related Product Information for anti-SYT1 antibody
Description: SYT1: synaptotagmin I. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse.
Function: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed:23999003).
Subunit Structure: Homotetramer (Probable). Interacts with SCAMP5, STON2, SV2A, SV2B, SV2C and RIMS1. Forms a complex with SV2B, syntaxin 1 and SNAP25 (By similarity). Interacts with PRRT2 (By similarity).
Similarity: The first C2 domain mediates Ca2+-dependent phospholipid binding.The second C2 domain mediates interaction with SV2A and probably with STN2. Belongs to the synaptotagmin family.
Function: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed:23999003).
Subunit Structure: Homotetramer (Probable). Interacts with SCAMP5, STON2, SV2A, SV2B, SV2C and RIMS1. Forms a complex with SV2B, syntaxin 1 and SNAP25 (By similarity). Interacts with PRRT2 (By similarity).
Similarity: The first C2 domain mediates Ca2+-dependent phospholipid binding.The second C2 domain mediates interaction with SV2A and probably with STN2. Belongs to the synaptotagmin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 60 kDa
Predicted: 48 kDa
Predicted: 48 kDa
NCBI Official Full Name
synaptotagmin-1 isoform 1
NCBI Official Synonym Full Names
synaptotagmin 1
NCBI Official Symbol
SYT1
NCBI Official Synonym Symbols
P65; SYT; SVP65
NCBI Protein Information
synaptotagmin-1
UniProt Protein Name
Synaptotagmin-1
Protein Family
UniProt Gene Name
SYT1
UniProt Synonym Gene Names
SVP65; SYT; SytI
NCBI Description
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]
Uniprot Description
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed:23999003).
Research Articles on SYT1
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Product Notes
The SYT1 syt1 (Catalog #AAA9602735) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SYT1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SYT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the SYT1 syt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SYT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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