Immunofluorescence (IF)
(Immunofluorescence staining of SDC4 in Hela cells or mouse splenocytes. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse SDC4 monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Rabbit anti-Mouse Syndecan-4 Monoclonal Antibody | anti-SDC4 antibody
Recombinant Anti-Syndecan-4 Antibody, Rabbit Monoclonal
Gene Names
Sdc4; Synd4; AA959608; AW108331; ryudocan; syndecan-4
Reactivity
Mouse
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A
Synonyms
Syndecan-4; Monoclonal Antibody; Recombinant Anti-Syndecan-4 Antibody; Rabbit Monoclonal; Syndecan-4/SDC4 Antibody; Rabbit MAb; syndecan 4; Anti-AA959608 Antibody; Anti-AW108331 Antibody; Anti-ryudocan Antibody; Anti-Synd4 Antibody; Anti-syndecan-4 Antibody; anti-SDC4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
047
Specificity
Mouse Syndecan-4/SDC4.
No cross-reactivity in ELISA with Mouse SDC1
No cross-reactivity in ELISA with Mouse SDC1
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SDC4 antibody
Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
IHC-P: 1:100-1:500
ICC/IF: 1:20-1:100
ICC/IF: 1:20-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse Syndecan-4/SDC4 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Syndecan-4/SDC4 (rM Syndecan-4/SDC4).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of SDC4 in Hela cells or mouse splenocytes. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse SDC4 monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of SDC4 in Hela cells or mouse splenocytes. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse SDC4 monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SDC4 in mouse stomach with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SDC4 in mouse stomach with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SDC4 in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SDC4 in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-SDC4 antibody
SDC4 (Syndecan-4), also known as Syn4, is a transmembrane heparan sulfate proteoglycan that co-operates with integrins during cell-matrix interactions for the assembly of focal adhesions and actin stress fibers and in the phosphorylation of focal adhesion kinase (FAK) on Tyr397. Syndecan-4 plays roles in the formation of focal adhesions and stress fibers. The cytoplasmic domain of syndecan-4 interacts with several signalling and structural proteins, and both extracellular and cytoplasmic domains are necessary for regulated activation of associated transmembrane receptors. Syndecan-4/SDC4 is a heparan sulfate proteoglycan and works as a coreceptor for various growth factors. SDC4 deficiency limits neointimal formation after vascular injury by regulating vascular smooth muscle cells (VSMCs) proliferation and vascular progenitor cells (VPCs) mobilization. Therefore, SDC4 may be a novel therapeutic target for preventing arterial restenosis after angioplasty.
References
Ikesue M, et al. (2011) Syndecan-4 deficiency limits neointimal formation after vascular injury by regulating vascular smooth muscle cell proliferation and vascular progenitor cell mobilization. Arterioscler Thromb Vasc Biol. 31(5): 1066-74.Saoncella S, et al. (2004) Syndecan-4 regulates ATF-2 transcriptional activity in a Rac1-dependent manner. J Biol Chem. 279(45): 47172-6.Bass MD, et al. (2002) Cytoplasmic interactions of syndecan-4 orchestrate adhesion receptor and growth factor receptor signalling. Biochem J. 368(Pt 1): 1-15.Couchman JR, et al. (1999) Syndecan-4 and integrins: combinatorial signaling in cell adhesion. J Cell Sci. 112 (Pt 20): 3415-20.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,482 Da
NCBI Official Full Name
syndecan-4
NCBI Official Synonym Full Names
syndecan 4
NCBI Official Symbol
Sdc4
NCBI Official Synonym Symbols
Synd4; AA959608; AW108331; ryudocan; syndecan-4
NCBI Protein Information
syndecan-4; ryudocan core protein
UniProt Protein Name
Syndecan-4
Protein Family
UniProt Gene Name
Sdc4
UniProt Synonym Gene Names
SYND4
UniProt Entry Name
SDC4_MOUSE
Uniprot Description
Function: Cell surface proteoglycan that bears heparan sulfate.
Research Articles on SDC4
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Product Notes
The SDC4 sdc4 (Catalog #AAA8112003) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Syndecan-4 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Syndecan-4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). IHC-P: 1:100-1:500 ICC/IF: 1:20-1:100. Researchers should empirically determine the suitability of the SDC4 sdc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Syndecan-4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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