Syndecan-4 Active Protein | SDC4 active protein

Syndecan-4 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122952

• Gene Names: Sdc4; Synd4; AA959608; AW108331; ryudocan; syndecan-4
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Syndecan-4; Syndecan-4 Protein; Mouse; Recombinant (hFc Tag); Mouse Syndecan-4/SDC4 Protein (Fc Tag); AA959608 Protein; AW108331 Protein; ryudocan Protein; Synd4 Protein; syndecan-4 Protein; syndecan 4; SDC4 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val146

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 24
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse SDC4 (O35988)(Met1-Val146) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human MDK at 10ug/ml (100uL/well) can bind mouse SDC4-Fc with a linear range of 0.16-1.25ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human MDK at 10 ug/ml (100 ul/well) can bind mouse SDC4-Fc with a linear range of 0.16-1.25 ug/ml.)

Related Product Information for SDC4 active protein

Background: SDC4 (Syndecan-4), also known as Syn4, is a transmembrane heparan sulfate proteoglycan that co-operates with integrins during cell-matrix interactions for the assembly of focal adhesions and actin stress fibers and in the phosphorylation of focal adhesion kinase (FAK) on Tyr397. Syndecan-4 plays roles in the formation of focal adhesions and stress fibers. The cytoplasmic domain of syndecan-4 interacts with several signalling and structural proteins, and both extracellular and cytoplasmic domains are necessary for regulated activation of associated transmembrane receptors. Syndecan-4/SDC4 is a heparan sulfate proteoglycan and works as a coreceptor for various growth factors. SDC4 deficiency limits neointimal formation after vascular injury by regulating vascular smooth muscle cells (VSMCs) proliferation and vascular progenitor cells (VPCs) mobilization. Therefore, SDC4 may be a novel therapeutic target for preventing arterial restenosis after angioplasty.

References

Ikesue M, et al. (2011) Syndecan-4 deficiency limits neointimal formation after vascular injury by regulating vascular smooth muscle cell proliferation and vascular progenitor cell mobilization. Arterioscler Thromb Vasc Biol. 31(5): 1066-74.Saoncella S, et al. (2004) Syndecan-4 regulates ATF-2 transcriptional activity in a Rac1-dependent manner. J Biol Chem. 279(45): 47172-6.Bass MD, et al. (2002) Cytoplasmic interactions of syndecan-4 orchestrate adhesion receptor and growth factor receptor signalling. Biochem J. 368(Pt 1): 1-15.Couchman JR, et al. (1999) Syndecan-4 and integrins: combinatorial signaling in cell adhesion. J Cell Sci. 112 (Pt 20): 3415-20.

NCBI and Uniprot Product Information

• NCBI GI #: 6755442
• NCBI GeneID: 20971
• NCBI Accession #: NP_035651.1
• NCBI GenBank Nucleotide #: NM_011521.2
• UniProt Accession #: O35988
• Molecular Weight: 21,482 Da
• NCBI Official Full Name: syndecan-4
• NCBI Official Synonym Full Names: syndecan 4
• NCBI Official Symbol: Sdc4
• NCBI Official Synonym Symbols: Synd4; AA959608; AW108331; ryudocan; syndecan-4
• NCBI Protein Information: syndecan-4; ryudocan core protein
• UniProt Protein Name: Syndecan-4
• Protein Family: Syndecan
• UniProt Gene Name: Sdc4
• UniProt Synonym Gene Names: SYND4
• UniProt Entry Name: SDC4_MOUSE

Uniprot Description

Function: Cell surface proteoglycan that bears heparan sulfate.