Mouse Synaptobrevin-3 Monoclonal Antibody | anti-VAMP3 antibody

Mouse Anti Human Synaptobrevin-3

Cat. Num. AAA140316

• Gene Names: VAMP3; CEB
• Applications: ELISA, Western Blot
• Purity: VAMP3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Synaptobrevin-3; Monoclonal Antibody; Mouse Anti Human Synaptobrevin-3; VAMP3 Antibody; Mouse Anti Human; VAMP3; VAMP-3; Cellubrevin; Vesicle-Associated Membrane Protein 3; CEB; SYB3; anti-VAMP3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b heavy chain and kappa light chain
• Clone Number: Pk2A2AT
• Purity/Purification: VAMP3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 100

• Applicable Applications for anti-VAMP3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: VAMP3 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
• Immunogen: Anti-human VAMP3 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human VAMP3 amino acids 1-77 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-VAMP3 antibody

Introduction: VAMP3 is present in recycling endosomes and endosome-derived vesicles. VAMP3 has been implicated in recycling of transferrin receptors to the plasma membrane, secretion of alpha-granules in platelets, recycling of T-cell receptors to the immunological synapses, and membrane trafficking during cell migration. VAMP-3 is present in human platelets and necessary for granule secretion. Synaptobrevins are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP3 high homology to other VAMPs in its broad tissue distribution and subcellular localization is shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation.

Product Categories/Family for anti-VAMP3 antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4759300
• NCBI GeneID: 9341
• NCBI Accession #: NP_004772.1
• NCBI GenBank Nucleotide #: NM_004781.3
• Molecular Weight: 11,309 Da
• NCBI Official Full Name: vesicle-associated membrane protein 3
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 3
• NCBI Official Symbol: VAMP3
• NCBI Official Synonym Symbols: CEB
• NCBI Protein Information: vesicle-associated membrane protein 3; VAMP-3; cellubrevin; synaptobrevin-3
• UniProt Protein Name: Vesicle-associated membrane protein 3
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP3
• UniProt Synonym Gene Names: SYB3; VAMP-3; CEB
• UniProt Entry Name: VAMP3_HUMAN

NCBI Description

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq, Jul 2008]

Uniprot Description

VAMP3: SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. Belongs to the synaptobrevin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: neuron projection; cell surface; intracellular membrane-bound organelle; clathrin-coated vesicle; integral to membrane; clathrin coated vesicle membrane; cytosol; secretory granule; SNARE complex; synaptic vesicle; recycling endosome; apical plasma membrane; plasma membrane; intracellular; cell junction

Molecular Function: SNAP receptor activity; protein binding; SNARE binding; syntaxin-1 binding

Biological Process: vesicle fusion; vesicle-mediated transport; exocytosis; neurotransmitter secretion; Golgi to plasma membrane protein transport; protein complex assembly; positive regulation of immunoglobulin secretion; retrograde transport, endosome to Golgi; positive regulation of receptor recycling; vesicle docking during exocytosis; calcium ion-dependent exocytosis