Mouse anti-Human SUMO-2/3 Monoclonal Antibody | anti-SUMO-2/3 antibody

SUMO-2/3 (HSMT3, Sentrin 2, Small Ubiquitin like Modifier 2, Small Ubiquitin like Modifier Protein 3, SMT3A, SMT3B, SMT3 Homolog 1, SMT3H1, SMT3 Homolog 2, SMT3H2, SMT3 Homolog, SMT3 Suppressor of mif two 3 Homolog 1, SMT3 Suppressor of mif two 3 Homolog

Cat. Num. AAA6124519

• Gene Names: SUMO2; HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: SUMO-2/3; Monoclonal Antibody; SUMO-2/3 (HSMT3; Sentrin 2; Small Ubiquitin like Modifier 2; Small Ubiquitin like Modifier Protein 3; SMT3A; SMT3B; SMT3 Homolog 1; SMT3H1; SMT3 Homolog 2; SMT3H2; SMT3 Homolog; SMT3 Suppressor of mif two 3 Homolog 1; SMT3 Suppressor of mif two 3 Homolog; HSMT3; SMT3 Suppressor of mif two 3 Homolog 2; SMT3 Suppressor of mif two 3 Homolog 3; Suppressor of mif two 3 Homolog 2; Suppressor of mif two 3 Homolog 3; Ubiquitin like Protein SMT3A; Ubiquitin like Protein SMT3B; anti-SUMO-2/3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: SPM572
• Specificity: Recognizes human SUMO-2/3. Shows broad species reactivity.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-SUMO-2/3 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: FC/FACS: 0.5-1ug/million cells; IP: 0.5-1ug/500ug protein lysate; IF: 0.5-1ug/ml; WB: 0.5-1ug/ml; IHC: 0.5-1ug/ml for 30 minutes at RT; Applications are based on unconjugated antibody.
• Immunogen: Recombinant human SUMO2 protein. Cellular Localization: Predominantly nuclear with some cytoplasmic
• Conjugate: FITC
• Positive Control: HeLa cells or breast carcinoma
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-paraffin human tonsil stained with SUMO-2 MAb (SPM572))

Product Categories/Family for anti-SUMO-2/3 antibody

Antibodies; Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 54792071
• NCBI GeneID: 6613
• NCBI Accession #: NP_001005849.1
• NCBI GenBank Nucleotide #: NM_001005849.1
• UniProt Accession #: P61956; P55854
• Molecular Weight: 10,871 Da
• NCBI Official Full Name: small ubiquitin-related modifier 2 isoform b
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 2
• NCBI Official Symbol: SUMO2
• NCBI Official Synonym Symbols: HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
• NCBI Protein Information: small ubiquitin-related modifier 2; sentrin 2; SMT3 homolog 2; ubiquitin-like protein SMT3A; ubiquitin-like protein SMT3B; SMT3 suppressor of mif two 3 homolog 2
• UniProt Protein Name: Small ubiquitin-related modifier 2
• UniProt Gene Name: SUMO2
• UniProt Synonym Gene Names: SMT3A; SMT3H2; SUMO-2; Smt3A
• UniProt Entry Name: SUMO2_HUMAN

NCBI Description

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

SUMO2: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Homotrimer (Potential). Crystal packing analysis suggests a possible trimeric assembly, of which the biological significance remains to be determined. Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins. Interacts with PELP1. Interacts with USP25; the interaction sumoylates USP25. Broadly expressed. Belongs to the ubiquitin family. SUMO subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: nucleoplasm; PML body; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: cellular protein metabolic process; protein sumoylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; post-translational protein modification