Western Blot (WB)
(Western Blot Positive WB detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate All lanes: ALIX antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 97, 97, 31 kDa Observed band size: 97 kDa)
PDCD6IP recombinant antibody
PDCD6IP Antibody
FC/FACS: 1:20-1:200
Western Blot (WB)
(Western Blot Positive WB detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate All lanes: ALIX antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 97, 97, 31 kDa Observed band size: 97 kDa)
Western Blot (WB)
(Western Blot Positive WB detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate All lanes: ALIX antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 97, 97, 31 kDa Observed band size: 97 kDa)
Flow Cytometry (FC/FACS)
(Overlay histogram showing Jurkat cells stained with (red line) at 1?50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing Jurkat cells stained with (red line) at 1?50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]
Uniprot Description
Alix: an adaptor protein that may regulate the function of receptor and cytoskeleton-associated tyrosine kinases. A class E vacuolar protein sorting (VPS) factor involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. May play a role in the regulation of both apoptosis and cell proliferation.
Protein type: Apoptosis; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 3p22.3
Cellular Component: focal adhesion; membrane; cytoplasm; melanosome; microtubule organizing center; immunological synapse; cytosol; vesicle
Molecular Function: protein binding; protein homodimerization activity; proteinase activated receptor binding; SH3 domain binding; calcium-dependent protein binding
Biological Process: protein transport; cell separation during cytokinesis; viral reproduction; apoptosis; viral infectious cycle; mitotic metaphase plate congression; nuclear organization and biogenesis
Research Articles on PDCD6IP
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Product Notes
The PDCD6IP pdcd6ip (Catalog #AAA7136686) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDCD6IP Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDCD6IP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:500-1:5000 FC/FACS: 1:20-1:200. Researchers should empirically determine the suitability of the PDCD6IP pdcd6ip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDCD6IP, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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