SULT2A1 recombinant protein

SULT2A1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120814

• Gene Names: SULT2A1; HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
• Purity: >97% as determined by SDS-PAGE
• Synonyms: SULT2A1; SULT2A1 Protein; Human; Recombinant (His Tag); Human SULT2A1/STD Protein (His Tag); DHEA-ST Protein; DHEAS Protein; HST Protein; hSTa Protein; ST2 Protein; ST2A1 Protein; ST2A3 Protein; STD Protein; sulfotransferase family; cytosolic; 2A; dehydroepiandrosterone (DHEA)-preferring; member 1; SULT2A1 recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 500mM NaCl, 20% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser2-Glu285

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human SULT2A1 (NP_003158.2)(Ser 2-Glu 285) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SULT2A1 recombinant protein

Background: Hydroxysteroid sulfotransferase (SULT2A1) is a key enzyme in the testicular and hepatic metabolism of 5alpha-androstenone, which is a major component of the off-odor and off-flavor in pork known as boar taint. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. SULT2A1 is a sulfo-conjugating phase II enzyme expressed at very high levels in the liver and intestine, the two major first-pass metabolic tissues, and in the steroidogenic adrenal tissue. SULT2A1 acts preferentially on the hydroxysteroids dehydroepiandrosterone, testosterone/dihydrotestosterone, and pregnenolone and on cholesterol-derived amphipathic sterol bile acids.

References

Chatterjee, B. 2005, Methods Enzymol. 400:165-91. Liu,Y. et al., 2006, Chem Res Toxicol. 19 (11):1420-5. Sinclair, PA. et al., 2006, J Mol Endocrinol 36 (2):301-11. Yalcin, EB. et al., 2008, Drug Metab Lett 2 (3):198-204.

NCBI and Uniprot Product Information

• NCBI GI #: 29540545
• NCBI GeneID: 6822
• NCBI Accession #: NP_003158.2
• NCBI GenBank Nucleotide #: NM_003167.3
• UniProt Accession #: Q06520
• Molecular Weight: 33,780 Da
• NCBI Official Full Name: bile salt sulfotransferase
• NCBI Official Synonym Full Names: sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
• NCBI Official Symbol: SULT2A1
• NCBI Official Synonym Symbols: HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
• NCBI Protein Information: bile salt sulfotransferase; alcohol/hydroxysteroid sulfotransferase; bile-salt sulfotranasferase 2A1
• UniProt Protein Name: Bile salt sulfotransferase
• Protein Family: Bile salt sulfotransferase
• UniProt Gene Name: SULT2A1
• UniProt Synonym Gene Names: HST; STD; DHEA-ST; HST; ST2A1
• UniProt Entry Name: ST2A1_HUMAN

NCBI Description

This gene encodes a member of the sulfotransferase family. Sulfotransferases aid in the metabolism of drugs and endogenous compounds by converting these substances into more hydrophilic water-soluble sulfate conjugates that can be easily excreted. This protein catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands, and may have a role in the inherited adrenal androgen excess in women with polycystic ovary syndrome. [provided by RefSeq, Mar 2010]

Uniprot Description

SULT2A1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Belongs to the sulfotransferase 1 family.

Protein type: EC 2.8.2.14; Transferase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cytosol

Molecular Function: sulfotransferase activity; protein binding; bile-salt sulfotransferase activity

Biological Process: steroid metabolic process; bile acid catabolic process; xenobiotic metabolic process; digestion; sulfation; cellular lipid metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process