Western Blot (WB)
(Figure 1: Western blot analysis using NCOR1 mouse mAb against truncated Trx-NCOR1 recombinant protein (1).)
Mouse anti-Human NCOR1 Monoclonal Antibody | anti-NCOR1 antibody
NCOR1 Antibody
Gene Names
NCOR1; N-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Affinity-Chromatography
Synonyms
NCOR1; Monoclonal Antibody; NCOR1 Antibody; hN CoR; hNCoR; KIAA1047; N CoR; N Cor/SMRT corepressor Rip13; N CoR1; N-CoR; N-CoR1; NCOR 1; NCoR; Ncor1; NCOR1_HUMAN; Nuclear receptor co repressor 1; Nuclear receptor corepressor 1; Retinoid X receptor interacting protein 13; RIP13; Rxrip13; thyroid hormone and retinoic acid receptor associated corepressor 1; TRAC 1; TRAC1; anti-NCOR1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
NCOR1 antibody detects endogenous levels of total NCOR1
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
914
Applicable Applications for anti-NCOR1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF/ICC: 1:100-1:500
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF/ICC: 1:100-1:500
Immunogen
Purified recombinant fragment of human NCOR1 expressed in E. Coli
Subcellular Location
Nucleus.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using NCOR1 mouse mAb against truncated Trx-NCOR1 recombinant protein (1).)
Western Blot (WB)
(Figure 1: Western blot analysis using NCOR1 mouse mAb against truncated Trx-NCOR1 recombinant protein (1).)
Related Product Information for anti-NCOR1 antibody
Description: NCOR1: Nuclear receptor co-repressor 1. This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. An alternatively spliced transcript variant has been described, but its full length sequence has not been determined.
Function: Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. Participates in the transcriptional repressor activity produced by BCL6.
Subunit Structure: Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid receptor (TR) and the retinoid acid receptor (RAR) in the absence of ligand. Interacts directly with RARA; the interaction is facilitated with RARA trimethylation. Component of the N-Cor repressor complex, at least composed of CBFA2T3, HEXIM1, NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2. Interacts with ZBTB33; the interaction serves to recruit the N-CoR complex to promoter regions containing methylated CpG dinucleotides. Interacts with TRIM28 and KDM3A. Interacts (via the RD1 domain) with BAZ1A (via its N-terminal); the interaction corepresses a number of NCOR1-regulated genes. Interacts with BCL6, C1D, DACH1, HEXIM1, HDAC7, RORA, RORC, SAP30, SIAH2, SIN3A and SIN3B. May interact with DEAF1. Interacts with RXRA. Interacts with SETD5 (By similarity). Interacts with VDR (PubMed:28698609).
Post-translational Modifications: Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.
Similarity: The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity (By similarity). Belongs to the N-CoR nuclear receptor corepressors family.
Function: Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. Participates in the transcriptional repressor activity produced by BCL6.
Subunit Structure: Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid receptor (TR) and the retinoid acid receptor (RAR) in the absence of ligand. Interacts directly with RARA; the interaction is facilitated with RARA trimethylation. Component of the N-Cor repressor complex, at least composed of CBFA2T3, HEXIM1, NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2. Interacts with ZBTB33; the interaction serves to recruit the N-CoR complex to promoter regions containing methylated CpG dinucleotides. Interacts with TRIM28 and KDM3A. Interacts (via the RD1 domain) with BAZ1A (via its N-terminal); the interaction corepresses a number of NCOR1-regulated genes. Interacts with BCL6, C1D, DACH1, HEXIM1, HDAC7, RORA, RORC, SAP30, SIAH2, SIN3A and SIN3B. May interact with DEAF1. Interacts with RXRA. Interacts with SETD5 (By similarity). Interacts with VDR (PubMed:28698609).
Post-translational Modifications: Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.
Similarity: The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity (By similarity). Belongs to the N-CoR nuclear receptor corepressors family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 270 kDa
Predicted: 271 kDa
Predicted: 271 kDa
NCBI Official Full Name
nuclear receptor corepressor 1 isoform 2
NCBI Official Synonym Full Names
nuclear receptor corepressor 1
NCBI Official Symbol
NCOR1
NCBI Official Synonym Symbols
N-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109
NCBI Protein Information
nuclear receptor corepressor 1
UniProt Protein Name
Nuclear receptor corepressor 1
Protein Family
UniProt Gene Name
NCOR1
UniProt Synonym Gene Names
KIAA1047; N-CoR; N-CoR1
NCBI Description
This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.[provided by RefSeq, Jun 2010]
Uniprot Description
Mediates transcriptional repression by certain nuclear receptors (PubMed:20812024). Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. Participates in the transcriptional repressor activity produced by BCL6. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024).
Research Articles on NCOR1
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Product Notes
The NCOR1 ncor1 (Catalog #AAA9602825) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NCOR1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NCOR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the NCOR1 ncor1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCOR1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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