Rabbit anti-Human Amyloid beta A4 protein Polyclonal Antibody | anti-APP antibody

Amyloid beta A4 protein Polyclonal Antibody

Cat. Num. AAA9415676

• Gene Names: APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: Amyloid beta A4 protein; Polyclonal Antibody; Amyloid beta A4 protein Polyclonal Antibody; ABPP APPI APP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide CVAP PreA4 Protease nexin-II PN-II; anti-APP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total Amyloid beta A4 protein polyclonal antibody.
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 770

• Applicable Applications for anti-APP antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:20 - 1:200
• Immunogen: Recombinant Human Amyloid beta A4 protein
• Immunogen Type: Protein
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embeded human colorectal carcinoma using at dilution of 1:100.)

Related Product Information for anti-APP antibody

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity By similarity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu2+-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoformsthat contain the BPTI domain possess protease inhibitor activity.Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Ref.36 Ref.63 Ref.65 Ref.88 Ref.89 Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity.Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu2+ and Fe3+ to Cu+ and Fe2+, respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Ref.36 Ref.63 Ref.65 Ref.88 Ref.89 Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain By similarity. Ref.36 Ref.63 Ref.65 Ref.88 Ref.89 The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. Ref.36 Ref.63 Ref.65 Ref.88 Ref.89 N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). Ref.36 Ref.63 Ref.65 Ref.88 Ref.89

Product Categories/Family for anti-APP antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4502167
• NCBI GeneID: 351
• NCBI Accession #: NP_000475.1
• NCBI GenBank Nucleotide #: NM_000484.3
• UniProt Accession #: P05067; P09000; P78438; Q13764; Q13778; Q13793; Q16011; B2R5V1; B4DII8; D3DSD1; D3DSD2; D3DSD3
• Molecular Weight: 84,521 Da
• NCBI Official Full Name: amyloid beta A4 protein isoform a
• NCBI Official Synonym Full Names: amyloid beta precursor protein
• NCBI Official Symbol: APP
• NCBI Official Synonym Symbols: AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• NCBI Protein Information: amyloid beta A4 protein
• UniProt Protein Name: Amyloid beta A4 protein
• Protein Family: Amyloid beta A4 protein
• UniProt Gene Name: APP
• UniProt Synonym Gene Names: A4; AD1; APP; CVAP; PN-II; S-APP-alpha; S-APP-beta; AICD-59; AID(59); AICD-57; AID(57); AICD-50; AID(50)
• UniProt Entry Name: A4_HUMAN

Uniprot Description

APP: a cell surface receptor that influences neurite growth, neuronal adhesion and axonogenesis. Cleaved by secretases to form a number of peptides, some of which bind to the acetyltransferase complex Fe65/TIP60 to promote transcriptional activation. The Abeta peptide is released from the cell, its extracellular deposition and accumulation form the main components of amyloid plaques in Alzheimer's disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis. Can promote transcription activation through binding to Fe65-Tip60 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(O) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. Induces a RAGE-dependent pathway that activates p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, JIP1, SHC1 and, NUMB and DAB1. Binding to DAB1 inhibits its serine phosphorylation. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Amyloid beta-42 binds nAChRA7 in hippocampal neurons. Beta-amyloid associates with HADH2. Soluble APP binds, via its N-terminal head, to FBLN1. Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. 10 isoforms of the human protein are produced by alternative splicing. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Belongs to the APP family.

Protein type: Apoptosis; Cell surface; Membrane protein, integral; Receptor, misc.; Transcription factor

Chromosomal Location of Human Ortholog: 21q21.3

Cellular Component: axon; cell surface; cytoplasm; cytosol; dendritic shaft; dendritic spine; endosome; extracellular region; extracellular space; Golgi apparatus; integral to membrane; integral to plasma membrane; intracellular membrane-bound organelle; lipid raft; nuclear envelope lumen; perinuclear region of cytoplasm; plasma membrane; receptor complex; synapse; trans-Golgi network membrane

Molecular Function: DNA binding; enzyme binding; identical protein binding; protein binding; PTB domain binding; receptor binding; serine-type endopeptidase inhibitor activity

Biological Process: adult locomotory behavior; axon cargo transport; axon midline choice point recognition; axonogenesis; cellular copper ion homeostasis; cellular process; cellular protein metabolic process; collateral sprouting in the absence of injury; dendrite development; endocytosis; extracellular matrix organization and biogenesis; innate immune response; ionotropic glutamate receptor signaling pathway; locomotory behavior; mating behavior; mRNA polyadenylation; neurite development; neuron apoptosis; neuron remodeling; platelet degranulation; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of epidermal growth factor receptor activity; regulation of multicellular organism growth; regulation of synapse structure and activity; regulation of translation; visual learning

Disease: Alzheimer Disease