Western Blot (WB)
(WB: 3T3/A31 cell lysates unstimulated (lane 1) and stimulated with PDGF (lane 2) were probed with S7972-13(2ug/ml). Arrow indicates phosphorylated STAT5 (92-94kD))
Mouse STAT5a,b Monoclonal Antibody | anti-STAT5a,b antibody
STAT5a,b, phosphorylated (Tyr694/Tyr699) (Signal Transducer and Activator of Transcription 5a,b) (AP)
Reactivity
Bovine, Human, Mouse, Rat, Sheep
Applications
Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
STAT5a; b; Monoclonal Antibody; phosphorylated (Tyr694/Tyr699) (Signal Transducer and Activator of Transcription 5a; b) (AP); anti-STAT5a; b antibody
Host
Mouse
Reactivity
Bovine, Human, Mouse, Rat, Sheep
Clonality
Monoclonal
Isotype
IgG
Clone Number
0.T.141
Specificity
Recognizes the phosphorylated tyrosine residues 694 of human STAT5A, 94kD and 699 of STAT5B, 92kD.
Species Crossreactivity: Rat, Mouse, Bovine and Sheep.
Species Crossreactivity: Rat, Mouse, Bovine and Sheep.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-STAT5a,b antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
WB: 1:1000 detected phospho-STAT5A/B in A431 cells stimulated with EGF. The lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with S7972-13. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
WB: 1:1000 detected phospho-STAT5A/B in A431 cells stimulated with EGF. The lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with S7972-13. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunogen
Synthetic phospho-peptide, KAVDG[pY]VKPQIK, corresponding to aa689-700 of STAT5A and 694-705 of STAT5B, conjugated to KLH.
Conjugate
AP
Positive Control
IFN-alpha induced HeLa cells, GM-CSF treated TF-1 cells.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(WB: 3T3/A31 cell lysates unstimulated (lane 1) and stimulated with PDGF (lane 2) were probed with S7972-13(2ug/ml). Arrow indicates phosphorylated STAT5 (92-94kD))
Western Blot (WB)
(WB: 3T3/A31 cell lysates unstimulated (lane 1) and stimulated with PDGF (lane 2) were probed with S7972-13(2ug/ml). Arrow indicates phosphorylated STAT5 (92-94kD))
Product Categories/Family for anti-STAT5a,b antibody
References
1. Darnell, J. E., et al., Science 264: 1415-1421, 1994. 2. David, M., et al., Science 269: 1721-1723, 1995. 3. Kirken, R.A., et al., J. Biol. Chem. 272: 15459-15465, 1997.
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Product Notes
The STAT5a,b (Catalog #AAA6241552) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The STAT5a,b, phosphorylated (Tyr694/Tyr699) (Signal Transducer and Activator of Transcription 5a,b) (AP) reacts with Bovine, Human, Mouse, Rat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's STAT5a,b can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. WB: 1:1000 detected phospho-STAT5A/B in A431 cells stimulated with EGF. The lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with S7972-13. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Researchers should empirically determine the suitability of the STAT5a,b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT5a,b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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