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Western Blot (WB) (Western blot analysis of extracts from UT-7 cells, untreated or treated with erythropoietin (EPO; 3 units/ml for 5 min), TF-1 cells, untreated or treated with Human Granulocyte Macrophage Colony Stimulating Factor 100ng/ml for 10 min), and NK-92 cells, untreated or treated with Human Interleukin-2 100ng/ml for 10 min), using Phospho-Stat5 (Tyr694) antibody or total Stat5 antibody.)

Rabbit anti-Human, Mouse STAT 5 Monoclonal Antibody | anti-Stat92E antibody

STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Mammary Gland Factor, MGF, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Transcription Factor STAT5A)

Gene Names
Stat92E; CG4257; d-STAT; D-stat; D-Stat; D-STAT; D-stat/stat92E; DmelCG4257; DmSTAT; DRODSRC; DSRC; dstat; dSTAT; Dstat; DSTAT; Dstat 92E; dSTAT92E; Dstat92E; dSTAT92E/marelle; l(3)06346; l(3)j6C8; mrl; mrL; mrl stat92E; pSTAT; SD-stat; stat; Stat; STAT; STAT 92E;
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Supernatant
Supernatant
Synonyms
STAT 5; Monoclonal Antibody; phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5; STAT5; Mammary Gland Factor; MGF; Signal Transducer and Activator of Transcription 5A; STAT5A; STAT5B; Transcription Factor STAT5A); Anti -STAT 5; anti-Stat92E antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
10E49
Specificity
Recognizes human Phospho-Stat5 (Tyr694). Species Crossreactivity
Purity/Purification
Supernatant
Supernatant
Form/Format
Supplied in liquid 10mM HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
Applicable Applications for anti-Stat92E antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence (IC) and Flow Cytometry.
Immunogen
Synthetic peptide corresponding to residues surrounding Tyr694 of human Stat5a protein (KLH-coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from UT-7 cells, untreated or treated with erythropoietin (EPO; 3 units/ml for 5 min), TF-1 cells, untreated or treated with Human Granulocyte Macrophage Colony Stimulating Factor 100ng/ml for 10 min), and NK-92 cells, untreated or treated with Human Interleukin-2 100ng/ml for 10 min), using Phospho-Stat5 (Tyr694) antibody or total Stat5 antibody.)

Western Blot (WB) (Western blot analysis of extracts from UT-7 cells, untreated or treated with erythropoietin (EPO; 3 units/ml for 5 min), TF-1 cells, untreated or treated with Human Granulocyte Macrophage Colony Stimulating Factor 100ng/ml for 10 min), and NK-92 cells, untreated or treated with Human Interleukin-2 100ng/ml for 10 min), using Phospho-Stat5 (Tyr694) antibody or total Stat5 antibody.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of TF-1 cells, untreated (blue) or GM-CSF treated (green), using Phospho-Stat5 (Tyr694) antibody.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of TF-1 cells, untreated (blue) or GM-CSF treated (green), using Phospho-Stat5 (Tyr694) antibody.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of A-431 cells, EGF-treated (left) or untreated (right), using Phospho-Stat5 (Tyr694) (green) and Pan-Keratin antibody.)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of A-431 cells, EGF-treated (left) or untreated (right), using Phospho-Stat5 (Tyr694) (green) and Pan-Keratin antibody.)
Related Product Information for anti-Stat92E antibody
Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).
Product Categories/Family for anti-Stat92E antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
86,415 Da
NCBI Official Full Name
Signal-transducer and activator of transcription protein at 92E, isoform M
NCBI Official Synonym Full Names
Signal-transducer and activator of transcription protein at 92E
NCBI Official Symbol
Stat92E
NCBI Official Synonym Symbols
CG4257; d-STAT; D-stat; D-Stat; D-STAT; D-stat/stat92E; DmelCG4257; DmSTAT; DRODSRC; DSRC; dstat; dSTAT; Dstat; DSTAT; Dstat 92E; dSTAT92E; Dstat92E; dSTAT92E/marelle; l(3)06346; l(3)j6C8; mrl; mrL; mrl stat92E; pSTAT; SD-stat; stat; Stat; STAT; STAT 92E;
NCBI Protein Information
CG4257 gene product from transcript CG4257-RH; CG4257-PB; CG4257-PC; CG4257-PE; CG4257-PF; CG4257-PG; CG4257-PH; CG4257-PI; CG4257-PJ; CG4257-PK; CG4257-PL; CG4257-PM; Signal-transducer and activator of transcription protein; Signal-transducer and activator of transcription protein at 92E; Stat92E-PB; Stat92E-PC; Stat92E-PE; Stat92E-PF; Stat92E-PG; Stat92E-PH; Stat92E-PI; Stat92E-PJ; Stat92E-PK; Stat92E-PL; Stat92E-PM; marelle; signal transducer and activator of transcription; signal transducers and activator of transcription
UniProt Protein Name
Signal transducer and transcription activator
UniProt Gene Name
Stat92E
UniProt Synonym Gene Names
MARE; mrL; Stat; d-STAT
UniProt Entry Name
STAT_DROME

Uniprot Description

STAT5A: Signal transduction and activation of transcription. Plays an important role in the segmental pattern formation in the early embryo by activating specific stripes of pair rule gene expression. The Janus kinase-STAT pathway is connected to drosophila early development

Protein type: Motility/polarity/chemotaxis; DNA-binding; Oncoprotein; Transcription factor

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; signal transducer activity; DNA binding; transcription factor activity

Biological Process: oogenesis; negative regulation of Wnt receptor signaling pathway; lymph gland hemopoiesis; somatic stem cell maintenance; primary sex determination; stem cell division; compound eye development; defense response; signal transduction; negative regulation of antimicrobial humoral response; segment specification; regulation of JAK-STAT cascade; regulation of transcription, DNA-dependent; hemocyte proliferation; positive regulation of cell proliferation; somatic stem cell division; hemopoiesis; epithelial cell fate determination, open tracheal system; nervous system development; germ-line stem cell division; imaginal disc-derived wing morphogenesis; larval somatic muscle development; stem cell maintenance; oocyte localization during germarium-derived egg chamber formation; germ-line stem cell maintenance; ovarian follicle cell stalk formation; JAK-STAT cascade; humoral immune response; border follicle cell migration; sex determination; long-term memory; open tracheal system development; cell dedifferentiation; lamellocyte differentiation; hindgut morphogenesis; positive regulation of defense response to virus by host; eye-antennal disc morphogenesis; blastoderm segmentation

Research Articles on Stat92E

Similar Products

Product Notes

The Stat92E stat92e (Catalog #AAA632022) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Mammary Gland Factor, MGF, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Transcription Factor STAT5A) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's STAT 5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence (IC) and Flow Cytometry. Researchers should empirically determine the suitability of the Stat92E stat92e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT 5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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