Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SSR2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate)
Mouse anti-Human SSR2 Monoclonal Antibody | anti-SSR2 antibody
SSR2 antibody
Gene Names
SSR2; TLAP; HSD25; TRAPB; TRAP-BETA
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
SSR2; Monoclonal Antibody; SSR2 antibody; Tanslocon-associated protein subunit beta precursor; HSD25; TLAP; TRAP-BETA; TRAPB; anti-SSR2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT31G6
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
183
Applicable Applications for anti-SSR2 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Recommended starting dilution is 1:500.
Antigen Species
Human
Immunogen
Recombinant human SSR2 (18-149aa) purified from E. coli
Clone Note
Anti-human SSR2 mAb, clone AT31G6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SSR2 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SSR2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate)
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SSR2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate)
Immunofluorescence (IF)
(ICC/IF analysis of SSR2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SSR2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of SSR2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SSR2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-SSR2 antibody
SSR2 belongs to the TRAP-beta family. SSR is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. The SSR consists of 2 subunits, a 34kD glycoprotein (alpha-SSR or SSR1) and a 22kD glycoprotein (beta-SSR or SSR2)..
Product Categories/Family for anti-SSR2 antibody
References
Chinen, K., et al. (1995) Cytogenet Cell Genet 70 (3-4): 215-7; Sikorska, M., et al. (2008) J Neurosci Res 86 (8): 1680-93
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,135 Da
NCBI Official Full Name
translocon-associated protein subunit beta
NCBI Official Synonym Full Names
signal sequence receptor subunit 2
NCBI Official Symbol
SSR2
NCBI Official Synonym Symbols
TLAP; HSD25; TRAPB; TRAP-BETA
NCBI Protein Information
translocon-associated protein subunit beta
UniProt Protein Name
Translocon-associated protein subunit beta
Protein Family
UniProt Gene Name
SSR2
UniProt Synonym Gene Names
TRAPB; TRAP-beta; SSR-beta
NCBI Description
The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein (alpha-SSR or SSR1) and a 22-kD glycoprotein (beta-SSR or SSR2). The human beta-signal sequence receptor gene (SSR2) maps to chromosome bands 1q21-q23. [provided by RefSeq, Jul 2008]
Uniprot Description
TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.
Research Articles on SSR2
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Product Notes
The SSR2 ssr2 (Catalog #AAA200285) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SSR2 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SSR2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the SSR2 ssr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SSR2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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