Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SSB protein (Right) or empty vector (Left) detected with SSB antibody)
Mouse SSB Monoclonal Antibody | anti-SSB antibody
SSB antibody
Applications
Immunohistochemistry, Western Blot
Purity
SSB antibody was purified by affinity chromatography.
Synonyms
SSB; Monoclonal Antibody; SSB antibody; Monoclonal SSB; Anti-SSB; Sjogren Syndrome Antigen B; Autoantigen La; LARP3; La; anti-SSB antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG3
Clone Number
9D6
Purity/Purification
SSB antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
162
Applicable Applications for anti-SSB antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50
WB: 1:2000
WB: 1:2000
Biological Significance
SSB is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(OH) elements of newly RNA polymerase III-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. SSB protein was origin ally defined by its reactivity with autoantibodies from patients with Sjogren syndrome and systemic lupus erythematosus.
Cross-Reactivity
Human, Monkey, Dog
Immunogen
SSB antibody was raised in mouse using a protein expressed in 293T cell transfected with human SSB expression vector as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SSB protein (Right) or empty vector (Left) detected with SSB antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SSB protein (Right) or empty vector (Left) detected with SSB antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of SSB protein in paraffin embedded Human Kidney tissue using SSB antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of SSB protein in paraffin embedded Human Kidney tissue using SSB antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SSB protein using SSB antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SSB protein using SSB antibody)
Related Product Information for anti-SSB antibody
Mouse monoclonal SSB antibody
Product Categories/Family for anti-SSB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,056 Da
NCBI Official Full Name
Ssb
NCBI Official Symbol
ssb
NCBI Protein Information
single-stranded DNA binding protein
UniProt Protein Name
Single-stranded DNA-binding protein
Protein Family
UniProt Gene Name
ssb
UniProt Entry Name
SSB_BPP1
Uniprot Description
This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair ().
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Product Notes
The SSB ssb (Catalog #AAA834312) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SSB can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50 WB: 1:2000. Researchers should empirically determine the suitability of the SSB ssb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SSB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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