Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of HeLa and A431 cells using SMARCA4 (Red). Actin filaments have been labeled with AlexaFluor® 488 dye Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains nucleus.)
Mouse anti-Human, Rat SMARCA4 Monoclonal Antibody | anti-SMARCA4 antibody
SMARCA4 (Transcription Activator BRG1, ATP-dependent Helicase SMARCA4, BRG1-associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-beta, SWI/SNF-related Matrix-associated Actin-dependent
Gene Names
SMARCA4; BRG1; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A; SNF2-beta
Reactivity
Human, Rat
Applications
Immunocytochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
SMARCA4; Monoclonal Antibody; SMARCA4 (Transcription Activator BRG1; ATP-dependent Helicase SMARCA4; BRG1-associated Factor 190A; BAF190A; Mitotic Growth and Transcription Activator; Protein BRG-1; Protein Brahma Homolog 1; SNF2-beta; SWI/SNF-related Matrix-associated Actin-dependent; anti-SMARCA4 antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
13A219
Specificity
Recognizes human SMARCA4. Species Crossreactivity: rat.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Sequence Length
1647
Applicable Applications for anti-SMARCA4 antibody
Immunocytochemistry (ICC), Western Blot (WB)
Immunogen
Recombinant protein corresponding to human SMARCA4 fused to GST-tag.
Conjugate
FITC
Preparation and Storage
Store at -20 degree C.
Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of HeLa and A431 cells using SMARCA4 (Red). Actin filaments have been labeled with AlexaFluor® 488 dye Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains nucleus.)
Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of HeLa and A431 cells using SMARCA4 (Red). Actin filaments have been labeled with AlexaFluor® 488 dye Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains nucleus.)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. HeLa nuclear extract was probed with SMARCA4, clone 20C3.2 (0.5ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SMARCA4 (~184kD).)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. HeLa nuclear extract was probed with SMARCA4, clone 20C3.2 (0.5ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SMARCA4 (~184kD).)
Product Categories/Family for anti-SMARCA4 antibody
References
1. Schneppenheim, R., et al. (2010). Am J Hum Genet. 86(2):279-284. 2. Fujimoto, M., et al. (2010). Mol Biol Cell. 21(1):106-116. 3. Zager, R.A., and Johnson, A.C. (2009). Am J Physiol Renal Physiol. 296(5):F1032-1041. 4. Kenneth, N.S., et al. (2009). J Biol Chem. 294(7):4123-4131. 5. Voss, T.C., et al. (2009). J Cell Sci. 122(Pt 3):345-356. 6. Osipovich, O., et al. (2007). Nat Immunol. 8(8):809-816. 7. Dammer, E.B., Leon, A., and Sewer, M.B. (2007). Mol Endocrinol. 21(2):415-438. 8. Ramirez-Carrozzi, V.R., et al. (2006). Genes Dev. 20(3):282-296. 9. Sif, S, et al. (1998). Genes Dev. 12(18):2842- 2851. 10. Schnitzler, G., Sif, S., and Kingston, R.E. (1998). Cell. 94(1):17-27.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
184
NCBI Official Full Name
transcription activator BRG1 isoform B
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
NCBI Official Symbol
SMARCA4
NCBI Official Synonym Symbols
BRG1; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A; SNF2-beta
NCBI Protein Information
transcription activator BRG1
UniProt Protein Name
Transcription activator BRG1
Protein Family
UniProt Gene Name
SMARCA4
UniProt Synonym Gene Names
BAF190A; BRG1; SNF2B; SNF2L4; BAF190A
UniProt Entry Name
SMCA4_HUMAN
NCBI Description
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Research Articles on SMARCA4
Similar Products
Product Notes
The SMARCA4 smarca4 (Catalog #AAA6243595) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SMARCA4 (Transcription Activator BRG1, ATP-dependent Helicase SMARCA4, BRG1-associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-beta, SWI/SNF-related Matrix-associated Actin-dependent reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCA4 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB). Researchers should empirically determine the suitability of the SMARCA4 smarca4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCA4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
