Mouse SPHINGOSINE 1- PHOSPHATE RECEPTOR 1 Monoclonal Antibody | anti-S1PR1 antibody

MOUSE ANTI HUMAN SPHINGOSINE 1- PHOSPHATE RECEPTOR 1

Cat. Num. AAA219926

• Gene Names: S1PR1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: SPHINGOSINE 1- PHOSPHATE RECEPTOR 1; Monoclonal Antibody; MOUSE ANTI HUMAN SPHINGOSINE 1- PHOSPHATE RECEPTOR 1; anti-S1PR1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 2B9
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 382

• Applicable Applications for anti-S1PR1 antibody: Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul; Flow Cytometry: Maximum Dilution: 1/10; Immunofluorescence: Maximum Dilution: 1/500; Immunoprecipitation: Maximum Dilution: 1/300; Western Blotting: Maximum Dilution: 1/5000
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-S1PR1 antibody

Mouse anti Human Sphingosine 1-Phosphate Receptor 1, clone 2B9 recognizes S1P1, otherwise known as CD363/EDG-1, a bioactive lysophospholipid and inducible member of the G-protein coupled receptor superfamily (GPCR) which plays an important role in a variety of cellular processes. During inflammatory diseases such as atherosclerosis the phenotype switching of activated macrophages is influenced by the presence of particular cytokines within the microenvironment. These cells have been characterized as classically activated M1 macrophages, in response to pro-inflammatory molecules such as interferon gamma and lipopolysaccharide, or as alternatively activated M2 macrophages, in response to anti-inflammatory cytokines such as interleukin-4. Activation of S1P1 through its ligand S1P (Sphingosine 1-Phosphate) has been identified as an important promoter of anti-inflammatory M2 macrophages (Hughes et al. 2008). Apoptotic cells can also influence the pro-versus anti-inflammatory macrophage response, and S1P acts as a positive regulator for anti-apoptotic/pro-survival responses by binding to S1P1. Growing evidence suggests that the expression of S1P1 on macrophages may convey protection against apoptosis, at least in part, having implications for the pathogenesis of inflammatory diseases and cancer. Deviation in sphingosine 1-phosphate (S1P) signaling is important in many types of cancer. Analysis of S1P1 and SK1 receptors during the development of tamoxifen resistance in Estrogen Receptor (ER) breast cancer patients concurs that the expression levels of S1P1 correlates with tumor size and Progesterone Receptor (PR) status and that high S1P1 membrane expression is associated with shorter time to recurrence.

NCBI and Uniprot Product Information

• NCBI GI #: 13027636
• NCBI GeneID: 1901
• NCBI Accession #: NP_001391.2
• NCBI GenBank Nucleotide #: NM_001400.4
• UniProt Accession #: P21453; Q9BYY4; Q9NYN8; D3DT66
• Molecular Weight: 42,811 Da
• NCBI Official Full Name: sphingosine 1-phosphate receptor 1
• NCBI Official Synonym Full Names: sphingosine-1-phosphate receptor 1
• NCBI Official Symbol: S1PR1
• NCBI Official Synonym Symbols: EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362
• NCBI Protein Information: sphingosine 1-phosphate receptor 1; S1P receptor 1; S1P receptor Edg-1; endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; sphingosine 1-phosphate receptor EDG1; sphingosine 1-phosphate receptor Edg-1
• UniProt Protein Name: Sphingosine 1-phosphate receptor 1
• Protein Family: Sphingosine 1-phosphate receptor
• UniProt Gene Name: S1PR1
• UniProt Synonym Gene Names: CHEDG1; EDG1; S1P receptor 1; S1P1; S1P receptor Edg-1
• UniProt Entry Name: S1PR1_HUMAN

NCBI Description

The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq, Jul 2008]

Uniprot Description

EDG-1: a G protein-coupled receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Highly expressed in endothelial cells and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. Suggested to be involved in the processes that regulate the migration/differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. S1P-induced endothelial cell migration requires AKT1-mediated phosphorylation of the third intracellular loop. Seems to be coupled to the G(i) subclass of heteromeric G proteins.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: plasma membrane; integral to membrane; intrinsic to plasma membrane; endosome; lipid raft; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; sphingolipid binding; G-protein-coupled receptor binding

Biological Process: lamellipodium biogenesis; regulation of cell adhesion; endothelial cell differentiation; regulation of bone resorption; cell migration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of positive chemotaxis; negative regulation of stress fiber formation; positive regulation of smooth muscle cell proliferation; transmission of nerve impulse; chemotaxis; blood vessel maturation; neuron differentiation; G-protein coupled receptor protein signaling pathway; actin cytoskeleton reorganization; positive regulation of transcription from RNA polymerase II promoter; brain development; angiogenesis; G-protein signaling, adenylate cyclase inhibiting pathway; cell adhesion; regulation of bone mineralization; positive regulation of cell migration