Rabbit RRAS2 Polyclonal Antibody | anti-RRAS2 antibody

RRAS2 Polyclonal Antibody

Cat. Num. AAA3011934

• Gene Names: RRAS2; NS12; TC21
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RRAS2; Polyclonal Antibody; RRAS2 Polyclonal Antibody; Ras-related protein R-Ras2; Ras-like protein TC21; Teratocarcinoma oncogene; TC21; anti-RRAS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RRAS2 polyclonal antibody detects endogenous levels of RRAS2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 204

• Applicable Applications for anti-RRAS2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human RRAS2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using RRAS2 antibody.)

Related Product Information for anti-RRAS2 antibody

H-, K- and N-Ras represent the prototype members of a family of small G proteins that are frequently activated to an oncogenic state in a wide variety of human tumors. Activation is due to point mutations at either position 12 or 61 within their coding sequence. Such mutations cause these proteins to be constitutively converted to their active GTP-bound, rather than the inactive GDP-bound, state. The related human R-Ras gene was initially cloned by low stringency hybridization methods. Position 38 and 87 (analogous to position 12 and 61 in H-Ras) mutants of R-Ras have been shown to be capable of activating oncogenic function. An additional member of the Ras oncogene family, designated TC 21 (or R-Ras-2) is most closely related to R-Ras. While wild type TC 21 does not exhibit transforming potential in vitro, mutant forms of TC 21 that possess amino acid substitutions analogous to those that activate Ras oncogenic potential, exhibit potent transforming activities comparable to the activity characteristic of the known oncogenic Ras proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 49065833
• NCBI GeneID: 22800
• UniProt Accession #: P62070
• Molecular Weight: ~ 23kDa
• NCBI Official Full Name: Ras-related protein R-Ras2
• NCBI Official Synonym Full Names: RAS related 2
• NCBI Official Symbol: RRAS2
• NCBI Official Synonym Symbols: NS12; TC21
• NCBI Protein Information: ras-related protein R-Ras2
• UniProt Protein Name: Ras-related protein R-Ras2
• Protein Family: Ras-related protein
• UniProt Gene Name: RRAS2
• UniProt Synonym Gene Names: TC21
• UniProt Entry Name: RRAS2_HUMAN

NCBI Description

This gene encodes a member of the R-Ras subfamily of Ras-like small GTPases. The encoded protein associates with the plasma membrane and may function as a signal transducer. This protein may play an important role in activating signal transduction pathways that control cell proliferation. Mutations in this gene are associated with the growth of certain tumors. Pseudogenes of this gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Uniprot Description

RRAS2: It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion. Defects in RRAS2 are a cause of susceptibility to ovarian cancer (OC). The term ovarian cancer defines common malignancies originating from ovarian tissue. Although many histologic types of ovarian tumors have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease. Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; Oncoprotein; G protein, monomeric, Ras; G protein, monomeric; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: focal adhesion; membrane; endoplasmic reticulum; plasma membrane

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: osteoblast differentiation; metabolic process; Ras protein signal transduction; positive regulation of cell migration