Immunofluorescence (IF)
(Fluorescent confocal image of SY5Y cells stained with phospho-Sox2- S246 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9206432 phospho-Sox2- S246 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-Sox2 immunosignal mainly to the nucleus.)
Rabbit phospho-Sox2 (S246) Polyclonal Antibody | anti-SOX2 antibody
phospho-Sox2 (S246) Antibody
DB~~1:500
Immunofluorescence (IF)
(Fluorescent confocal image of SY5Y cells stained with phospho-Sox2- S246 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9206432 phospho-Sox2- S246 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-Sox2 immunosignal mainly to the nucleus.)
Immunofluorescence (IF)
(Fluorescent confocal image of SY5Y cells stained with phospho-Sox2- S246 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9206432 phospho-Sox2- S246 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-Sox2 immunosignal mainly to the nucleus.)
Dot Blot (DB)
(Dot blot analysis of anti-phospho-Sox2-pS246 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Dot Blot (DB)
(Dot blot analysis of anti-phospho-Sox2-pS246 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
HMG-box (SOX) family of transcription factors involved in the
regulation of embryonic development and in the determination of
cell fate. The product of this gene is required for stem-cell
maintenance in the central nervous system, and also regulates gene
expression in the stomach. Mutations in this gene have been
associated with optic nerve hypoplasia and with syndromic
microphthalmia, a severe form of structural eye malformation. This
gene lies within an intron of another gene called SOX2 overlapping
transcript (SOX2OT).
1.Gen, Y., et al. Cancer Genet. Cytogenet. 202(2):82-93(2010)
2.Ji, J., et al. Hum. Pathol. 41(10):1438-1447(2010)
3.Fang, X., et al. OMICS 14(4):369-384(2010)
4.Sholl, L.M., et al. Am. J. Surg. Pathol. 34(8):1193-1198(2010)
5.Zhang, D., et al. BMC Med. Genet. 11, 116 (2010)
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
NCBI and Uniprot Product Information
NCBI Description
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
Uniprot Description
SOX2: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.
Protein type: Cell development/differentiation; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 3q26.3-q27
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus
Molecular Function: protein binding; miRNA binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; chromatin binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; tongue development; establishment and/or maintenance of chromatin architecture; somatic stem cell maintenance; endodermal cell fate specification; positive regulation of transcription, DNA-dependent; diencephalon morphogenesis; negative regulation of transcription from RNA polymerase II promoter; regulation of caspase activity; forebrain neuron differentiation; olfactory placode formation; detection of mechanical stimulus involved in equilibrioception; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; tissue regeneration; forebrain development; positive regulation of cell-cell adhesion; male genitalia development; response to wounding; adenohypophysis development; positive regulation of epithelial cell differentiation; negative regulation of osteoblast differentiation; cell cycle arrest; inner ear development; positive regulation of Notch signaling pathway; negative regulation of epithelial cell proliferation; positive regulation of neuroblast proliferation; inner ear morphogenesis; response to retinoic acid; neuron fate commitment; detection of mechanical stimulus involved in sensory perception of sound; osteoblast differentiation; negative regulation of neuron differentiation; eye development; pituitary gland development; regulation of gene expression; glial cell fate commitment; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; positive regulation of neuron differentiation; pigment biosynthetic process; alveolus development; retina morphogenesis in camera-type eye
Disease: Microphthalmia, Syndromic 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia
Research Articles on SOX2
Similar Products
Product Notes
The SOX2 sox2 (Catalog #AAA9206432) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The phospho-Sox2 (S246) Antibody reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's phospho-Sox2 (S246) can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Dot Blot (DB), ELISA (EIA). IF~~1:200 DB~~1:500. Researchers should empirically determine the suitability of the SOX2 sox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "phospho-Sox2 (S246), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
