Mouse Sodium-Iodide Symporter Monoclonal Antibody | anti-SLC5A5 antibody

Sodium-Iodide Symporter Antibody: HRP

Cat. Num. AAA801283

• Gene Names: SLC5A5; NIS; TDH1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Synonyms: Sodium-Iodide Symporter; Monoclonal Antibody; Sodium-Iodide Symporter Antibody: HRP; NIS; SLC5A5; solute carrier family 5; Na (+)I(-) cotransporter; anti-SLC5A5 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 14F
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 643

• Applicable Applications for anti-SLC5A5 antibody: Western Blot (WB)
• Application Notes: 1:1000 (WB)
• Conjugate: HRP
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)

Western Blot (WB)

(Western Blot analysis of Human thyroid lysate showing detection of Sodium Iodide Symporter protein using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)

Related Product Information for anti-SLC5A5 antibody

Background Info: Apparent mol. wt of 97kD, non-glycosylated version at 68kD. Other minor bands associated with hNIS at160kDa, and degradation products at ~30 kDa, and ~15kDa.

Scientific Background: The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3).

Product Categories/Family for anti-SLC5A5 antibody

Transporters

References

1. Dai G., Levy O., Carrasco N. (1996) Nature. 379(6564): 458-460. 2. Snabik P.A., et al. (1997) Endocrin. 138(8): 3555-3558. 3. Dohan O., et al. (2007) Proc Natl Acad Sci USA. 104(51): 20250-20255.

NCBI and Uniprot Product Information

• NCBI GI #: 4507035
• NCBI GeneID: 6528
• NCBI Accession #: NP_000444.1
• NCBI GenBank Nucleotide #: NM_000453.2
• UniProt Accession #: Q92911; O43702; Q2M335; Q9NYB6
• Molecular Weight: 68,666 Da
• NCBI Official Full Name: sodium/iodide cotransporter
• NCBI Official Synonym Full Names: solute carrier family 5 (sodium/iodide cotransporter), member 5
• NCBI Official Symbol: SLC5A5
• NCBI Official Synonym Symbols: NIS; TDH1
• NCBI Protein Information: sodium/iodide cotransporter; Na(+)/I(-) symporter; Na(+)/I(-) cotransporter; solute carrier family 5 (sodium iodide symporter), member 5
• UniProt Protein Name: Sodium/iodide cotransporter
• Protein Family: Sodium/iodide cotransporter
• UniProt Gene Name: SLC5A5
• UniProt Synonym Gene Names: NIS; Na(+)/I(-) cotransporter; Na(+)/I(-) symporter
• UniProt Entry Name: SC5A5_HUMAN

NCBI Description

This gene encodes a member of the sodium glucose cotransporter family. The encoded protein is responsible for the uptake of iodine in tissues such as the thyroid and lactating breast tissue. The iodine taken up by the thyroid is incorporated into the metabolic regulators triiodothyronine (T3) and tetraiodothyronine (T4). Mutations in this gene are associated with thyroid dyshormonogenesis 1.[provided by RefSeq, Sep 2009]

Uniprot Description

SLC5A5: Mediates iodide uptake in the thyroid gland. Defects in SLC5A5 are the cause of thyroid dyshormonogenesis 1 (TDH1). A disorder characterized by the inability of the thyroid to maintain a concentration difference of readily exchangeable iodine between the plasma and the thyroid gland, leading to congenital hypothyroidism. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: plasma membrane; integral to membrane; nucleus; vesicle

Molecular Function: iodide transmembrane transporter activity; sodium:iodide symporter activity

Biological Process: transport; iodide transport; ion transport; thyroid hormone generation; transmembrane transport

Disease: Thyroid Dyshormonogenesis 1