Ephrin A4 (EFNA4) Recombinant Protein | EFNA4 recombinant protein

Recombinant Ephrin A4 (EFNA4)

Cat. Num. AAA2009096

• Gene Names: Efna4; Epl4; EFL-4; LERK-4
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Ephrin A4 (EFNA4); Recombinant Ephrin A4 (EFNA4); EFNA4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-MRLLPLLRTV LWAALLGSRL PGCSSLRHPI YWNSSNPRLL RGDAVVELGF NDYLDIFCPH YESPGPPEGP ETFALYMVDW SGYEACTAEG ANAFQRWNCS MPFAPFSPVR FSEKIQRYTP FPLGFEFLPG ETYYYISVPT PESPGRCLRL QVSVCCKESG SSHESAHPVG SPGESGTSGW RGGHAPSPLC LLLLLLLPIL RLLRVL
• Sequence Length: 206

• Applicable Applications for EFNA4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Leu206 (Accession # O08542) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 110835739
• NCBI GeneID: 13639
• NCBI Accession #: NP_031936.2
• NCBI GenBank Nucleotide #: NM_007910.2
• UniProt Accession #: O08542; O55218
• Molecular Weight: 55.3kDa
• NCBI Official Full Name: ephrin-A4
• NCBI Official Synonym Full Names: ephrin A4
• NCBI Official Symbol: Efna4
• NCBI Official Synonym Symbols: Epl4; EFL-4; LERK-4
• NCBI Protein Information: ephrin-A4; EPH-related receptor tyrosine kinase ligand 4
• UniProt Protein Name: Ephrin-A4
• Protein Family: Ephrin
• UniProt Gene Name: Efna4
• UniProt Synonym Gene Names: Epl4; Eplg4; Lerk4; LERK-4
• UniProt Entry Name: EFNA4_MOUSE

Uniprot Description

EFNA4: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. May play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils. Belongs to the ephrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Ligand, receptor tyrosine kinase

Cellular Component: membrane; plasma membrane

Molecular Function: ephrin receptor binding

Biological Process: axon guidance; ephrin receptor signaling pathway; osteoclast differentiation; bone remodeling