Mouse anti-Human SLC11A2 / DMT1 Monoclonal Antibody | anti-SLC11A2 antibody

Mouse Monoclonal [clone 4C6] (IgG2a,k) to Human SLC11A2 / DMT1

Cat. Num. AAA246435

• Gene Names: SLC11A2; DCT1; DMT1; NRAMP2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: SLC11A2 / DMT1; Monoclonal Antibody; Mouse Monoclonal [clone 4C6] (IgG2a; k) to Human SLC11A2 / DMT1; Anti-SLC11A2 / DMT1 Antibody (clone 4C6) IHC-plus; SLC11A2; Divalent metal transporter 1; Divalent cation transporter 1; DMT-1; NRAMP 2; NRAMP2; Dct-1; DCT1; DMT1; Human SLC11A2; anti-SLC11A2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4C6
• Specificity: Human SLC11A2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.44 mg/ml (varies by lot)
• Sequence Length: 561

• Applicable Applications for anti-SLC11A2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western blot with the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: SLC11A2 (NP_000608, 1 a.a. ~ 66 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: SLC11A2 / DMT1 antibody was raised against sLC11A2 (NP_000608, 1 a.a. ~ 66 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SLC11A2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

ELISA (EIA)

(Detection limit for recombinant GST tagged SLC11A2 is 0.03 ng/ml as a capture antibody.)

Related Product Information for anti-SLC11A2 antibody

Solute carrier family 11, member 2 (SLCA2) also known as divalent metal ion transporter 1 (DMT1) imports Fe2+ via a proton-coupled, membrane potential dependent manner. It can also transport a variety of other divalent metal cations including Mn2+, Co2+, Cu2+, and Zn2+ but iron appears to be its most important physiological substrate.

Product Categories/Family for anti-SLC11A2 antibody

Family: Transporter; Subfamily: Natural resistance-associated macrophage protein (NRAMP)

NCBI and Uniprot Product Information

• NCBI GI #: 10835169
• NCBI GeneID: 4891
• NCBI Accession #: NP_000608.1
• NCBI GenBank Nucleotide #: NM_000617.2
• UniProt Accession #: P49281; O43288; O60932; O94801; Q498Z5; Q8IUD7; Q96J35; B3KT08; B4DK84; F5H741
• Molecular Weight: 61,048 Da
• NCBI Official Full Name: natural resistance-associated macrophage protein 2 isoform 3
• NCBI Official Synonym Full Names: solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2
• NCBI Official Symbol: SLC11A2
• NCBI Official Synonym Symbols: DCT1; DMT1; NRAMP2
• NCBI Protein Information: natural resistance-associated macrophage protein 2; DMT-1; NRAMP 2; divalent cation transporter 1; solute carrier family 11 member 2; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
• UniProt Protein Name: Natural resistance-associated macrophage protein 2
• Protein Family: Natural resistance-associated macrophage protein
• UniProt Gene Name: SLC11A2
• UniProt Synonym Gene Names: DCT1; DMT1; NRAMP2; NRAMP 2; DMT-1
• UniProt Entry Name: NRAM2_HUMAN

NCBI Description

This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2010]

Uniprot Description

SLC11A2: Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution. Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA). The disease is characterized by an abnormal hemoglobin content in the erythrocytes which are reduced in size. It may be hereditary or acquired. Mutations in SLC11A2 are associated with progressive liver iron overload and normal to moderately elevated serum ferritin levels. Belongs to the NRAMP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cell surface; lysosome; integral to plasma membrane; lysosomal membrane; late endosome membrane; early endosome; trans-Golgi network; recycling endosome; mitochondrial outer membrane; membrane; apical part of cell; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; late endosome; plasma membrane; vacuole; cytoplasmic vesicle; nucleus; brush border

Molecular Function: cadmium ion transmembrane transporter activity; nickel ion transmembrane transporter activity; inorganic cation transmembrane transporter activity; zinc ion binding; manganese ion transmembrane transporter activity; hydrogen ion transmembrane transporter activity; cadmium ion binding; nickel ion binding; vanadium ion transmembrane transporter activity; copper ion transmembrane transporter activity; protein binding; copper ion binding; manganese ion binding; iron ion binding; zinc ion transmembrane transporter activity; calcium ion transmembrane transporter activity; cobalt ion transmembrane transporter activity; ferrous iron transmembrane transporter activity; lead ion transmembrane transporter activity; cobalt ion binding; solute:hydrogen symporter activity

Biological Process: lead ion transport; caspase activation; erythrocyte development; cellular copper ion homeostasis; cellular iron ion homeostasis; dendrite morphogenesis; manganese ion transport; copper ion transport; response to manganese ion; nickel ion transport; response to cadmium ion; learning and/or memory; vanadium ion transport; detection of oxygen; ferrous iron transport; response to lead ion; response to hypoxia; response to iron ion; transmembrane transport; cobalt ion transport; heme biosynthetic process

Disease: Anemia, Hypochromic Microcytic, With Iron Overload 1