Mouse anti-Human NRAMP2 Monoclonal Antibody | anti-SLC11A2 antibody

NRAMP2 (Natural Resistance-associated Macrophage Protein 2, NRAMP 2, Divalent Cation Transporter 1, Divalent Metal Transporter 1, DMT-1, DCT1, DMT1, SLC11A2, OK/SW-cl.20)

Cat. Num. AAA643669

• Gene Names: SLC11A2; DCT1; DMT1; NRAMP2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NRAMP2; Monoclonal Antibody; NRAMP2 (Natural Resistance-associated Macrophage Protein 2; NRAMP 2; Divalent Cation Transporter 1; Divalent Metal Transporter 1; DMT-1; DCT1; DMT1; SLC11A2; OK/SW-cl.20); Anti -NRAMP2 (Natural Resistance-associated Macrophage Protein 2; anti-SLC11A2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4C6
• Specificity: Recognizes human SLC11A2.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-SLC11A2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Recombinant corresponding to aa1-66 from human SLC11A2 with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Testing Data

(Detection limit for recombinant GST tagged SLC11A2 is 0.03ng/ml as a capture antibody.)

Related Product Information for anti-SLC11A2 antibody

Solute carrier family 11, member 2 (SLCA2) also known as divalent metal ion transporter 1 (DMT1) imports Fe2+ via a proton-coupled, membrane potential dependent manner. It can also transport a variety of other divalent metal cations including Mn2+, Co2+, Cu2+, and Zn2+ but iron appears to be its most important physiological substrate. SLC11A2 has been demonstrated on the apical membrane of duodenal enterocytes, transferrin cycle endosomes in erythroid precursors, hepatocytes where it has been postulated to be involved in non-transferrin-bound iron uptake, and placenta where it has been implicated in transfer of iron from mother to fetus.

Product Categories/Family for anti-SLC11A2 antibody

Antibodies; Abs to Ion Transport

NCBI and Uniprot Product Information

• NCBI GI #: 295293178
• NCBI GeneID: 4891
• NCBI Accession #: NP_001167601.1
• NCBI GenBank Nucleotide #: NM_001174130.1
• UniProt Accession #: P49281; O43288; O60932; O94801; Q498Z5; Q8IUD7; Q96J35; B3KT08; B4DK84; F5H741
• Molecular Weight: 62,266 Da
• NCBI Official Full Name: natural resistance-associated macrophage protein 2 isoform 4
• NCBI Official Synonym Full Names: solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2
• NCBI Official Symbol: SLC11A2
• NCBI Official Synonym Symbols: DCT1; DMT1; NRAMP2
• NCBI Protein Information: natural resistance-associated macrophage protein 2; DMT-1; NRAMP 2; divalent cation transporter 1; solute carrier family 11 member 2; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
• UniProt Protein Name: Natural resistance-associated macrophage protein 2
• Protein Family: Natural resistance-associated macrophage protein
• UniProt Gene Name: SLC11A2
• UniProt Synonym Gene Names: DCT1; DMT1; NRAMP2; NRAMP 2; DMT-1
• UniProt Entry Name: NRAM2_HUMAN

NCBI Description

This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2010]

Uniprot Description

SLC11A2: Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution. Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA). The disease is characterized by an abnormal hemoglobin content in the erythrocytes which are reduced in size. It may be hereditary or acquired. Mutations in SLC11A2 are associated with progressive liver iron overload and normal to moderately elevated serum ferritin levels. Belongs to the NRAMP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cell surface; late endosome membrane; lysosome; lysosomal membrane; integral to plasma membrane; early endosome; trans-Golgi network; recycling endosome; mitochondrial outer membrane; membrane; apical part of cell; perinuclear region of cytoplasm; cytoplasm; late endosome; apical plasma membrane; plasma membrane; vacuole; cytoplasmic vesicle; nucleus; brush border

Molecular Function: cadmium ion transmembrane transporter activity; nickel ion transmembrane transporter activity; inorganic cation transmembrane transporter activity; zinc ion binding; manganese ion transmembrane transporter activity; hydrogen ion transmembrane transporter activity; cadmium ion binding; vanadium ion transmembrane transporter activity; nickel ion binding; copper ion transmembrane transporter activity; protein binding; copper ion binding; iron ion binding; manganese ion binding; zinc ion transmembrane transporter activity; calcium ion transmembrane transporter activity; cobalt ion transmembrane transporter activity; lead ion transmembrane transporter activity; ferrous iron transmembrane transporter activity; cobalt ion binding; solute:hydrogen symporter activity

Biological Process: lead ion transport; caspase activation; erythrocyte development; cellular copper ion homeostasis; cellular iron ion homeostasis; dendrite morphogenesis; manganese ion transport; copper ion transport; response to manganese ion; nickel ion transport; learning and/or memory; response to cadmium ion; detection of oxygen; vanadium ion transport; ferrous iron transport; response to lead ion; response to hypoxia; response to iron ion; cobalt ion transport; transmembrane transport; heme biosynthetic process

Disease: Anemia, Hypochromic Microcytic, With Iron Overload 1