Mouse anti-Human, Mouse SIRPA Monoclonal Antibody | anti-SIRPA antibody

SIRPA (BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, Tyrosine-protein Phosphatase Non-receptor Type Substrate 1, Brain Ig-like Molecule with Tyrosine-based Activation Motifs, CD172 Antigen-like Family Member A, Inhibitory Receptor SHPS-1, Macrophage Fusion Recepto

Cat. Num. AAA6154435

• Gene Names: SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SIRPA; Monoclonal Antibody; SIRPA (BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; Tyrosine-protein Phosphatase Non-receptor Type Substrate 1; Brain Ig-like Molecule with Tyrosine-based Activation Motifs; CD172 Antigen-like Family Member A; Inhibitory Receptor SHPS-1; Macrophage Fusion Recepto; anti-SIRPA antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4C7
• Specificity: Recognizes human PTPNS1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-SIRPA antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant protein corresponding to aa28-508 from human PTPNS1 (AAH26692) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: VAGEEELQVIQPDKSVSVAAGESAILHCTVTSLIPVGPIQWFRGAGPARELIYNQKEGHFPRVTTVSESTKRENMDFSISISNITPADAGTYYCVKFRKGSPDTEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGVVCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEPEKNAREITQVQSLDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (78.91kD).)

Western Blot (WB)

(PTPNS1 monoclonal antibody. Western Blot analysis of PTPNS1 expression in Raw 264.7.)

Western Blot (WB)

(PTPNS1 monoclonal antibody, Western Blot analysis of PTPNS1 expression in NIH/3T3.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PTPNS1 on NIH/3T3 cell. [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged PTPNS1 is ~0.1ng/ml as a capture antibody.)

Related Product Information for anti-SIRPA antibody

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Product Categories/Family for anti-SIRPA antibody

Antibodies; CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 20070654
• NCBI GeneID: 140885
• Molecular Weight: 54,852 Da
• NCBI Official Full Name: Homo sapiens signal-regulatory protein alpha, mRNA
• NCBI Official Synonym Full Names: signal regulatory protein alpha
• NCBI Official Symbol: SIRPA
• NCBI Official Synonym Symbols: BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type substrate 1
• Protein Family: Tyrosine-protein phosphatase non-receptor

NCBI Description

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]