Rabbit anti-Rat SIRP alpha Monoclonal Antibody | anti-SIRPA antibody

Recombinant Anti-SIRP alpha Antibody, Rabbit Monoclonal

Cat. Num. AAA8112881

• Gene Names: Sirpa; Bit; Ptpns1; SHPS-1
• Reactivity: Rat
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: SIRP alpha; Monoclonal Antibody; Recombinant Anti-SIRP alpha Antibody; Rabbit Monoclonal; SIRPA/SIRP alpha/CD172a Antibody; Rabbit MAb; signal-regulatory protein alpha; anti-SIRPA antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 103
• Specificity: Rat SIRPA/SIRP alpha/CD172a
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SIRPA antibody: Flow Cytometry (FC/FACS/FCM)
• Application Notes: FC/FACS/FCM: 1:25-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Rat SIRPA/SIRP alpha/CD172a Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Rat SIRPA/SIRP alpha/CD172a (rR SIRPA/SIRP alpha/CD172a).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-SIRPA antibody

Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

Product Categories/Family for anti-SIRPA antibody

Cancer Drug Targets

References

Timms JF. et al., 1999, Curr Biol. 9: 927-30. Stofega MR. et al., 2000, J Biol Chem. 275: 28222-9. Liu T. et al., 2005, J Proteome Res. 4: 2070-80. Wolf-Yadlin A. et al., 2007, Proc Natl Acad Sci. 104: 5860-5.

NCBI and Uniprot Product Information

• NCBI GI #: 31543529
• NCBI GeneID: 25528
• NCBI Accession #: NP_037148.2
• NCBI GenBank Nucleotide #: NM_013016.2
• UniProt Accession #: P97710; O08951; O70426; Q9QWI5
• Molecular Weight: 55,691 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type substrate 1
• NCBI Official Synonym Full Names: signal-regulatory protein alpha
• NCBI Official Symbol: Sirpa
• NCBI Official Synonym Symbols: Bit; Ptpns1; SHPS-1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type substrate 1
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type substrate 1
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: Sirpa
• UniProt Synonym Gene Names: Bit; Mfr; Ptpns1; Shps1; Sirp; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1
• UniProt Entry Name: SHPS1_RAT

Uniprot Description

SHPS1: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B. Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; protein phosphorylated amino acid binding

Biological Process: actin filament organization; cell migration; cell-matrix adhesion; cytoskeleton organization and biogenesis; hemopoietic progenitor cell differentiation; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of phagocytosis