Mouse anti-Human SIRP alpha Monoclonal Antibody | anti-SIRPA antibody

Anti-SIRP alpha Antibody (PE), Mouse Monoclonal

Cat. Num. AAA8105499

• Gene Names: SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: SIRP alpha; Monoclonal Antibody; Anti-SIRP alpha Antibody (PE); Mouse Monoclonal; SIRP alpha/CD172a Antibody (PE); Mouse MAb; signal-regulatory protein alpha; Anti-BIT Antibody; Anti-CD172A Antibody; Anti-MFR Antibody; Anti-MYD-1 Antibody; Anti-P84 Antibody; Anti-PTPNS1 Antibody; Anti-SHPS1 Antibody; Anti-SIRP Antibody; Anti-SIRP alpha Antibody; anti-SIRPA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 03
• Specificity: Human SIRPA/CD172A
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.1% sodium azide
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-SIRPA antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human SIRPA/CD172A protein
• Conjugation: PE
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SIRPA/CD172A (rh SIRPA/CD172A) and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of anti-human SIRPa (CD172a) on human whole blood monocytes. Human monocytess were stained with PE-anti human SIRPa (CD172a) (solid line) or PE-Mouse isotype control (dashed line).)

Related Product Information for anti-SIRPA antibody

Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

Product Categories/Family for anti-SIRPA antibody

Cancer Drug Targets

References

Timms JF. et al., 1999, Curr Biol. 9: 927-30. Stofega MR. et al., 2000, J Biol Chem. 275: 28222-9. Liu T. et al., 2005, J Proteome Res. 4: 2070-80. Wolf-Yadlin A. et al., 2007, Proc Natl Acad Sci. 104: 5860-5.

NCBI and Uniprot Product Information

• NCBI GI #: 18426911
• NCBI GeneID: 140885
• NCBI Accession #: NP_542970.1
• NCBI GenBank Nucleotide #: NM_080792.2
• UniProt Accession #: P78324; O00683; O43799; Q8N517; Q8TAL8; Q9H0Z2; Q9UDX2; Q9UIJ6; Q9Y4U9; A2A2E1; A8K411
• Molecular Weight: 54,967 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type substrate 1
• NCBI Official Synonym Full Names: signal-regulatory protein alpha
• NCBI Official Symbol: SIRPA
• NCBI Official Synonym Symbols: BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; OTTHUMP00000030001; OTTHUMP00000159968; OTTHUMP00000159969; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP sub
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type substrate 1
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: SIRPA
• UniProt Synonym Gene Names: BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1; Sirp-alpha-2
• UniProt Entry Name: SHPS1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq]

Uniprot Description

SHPS1: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B. Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Motility/polarity/chemotaxis; Cell adhesion; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: SH3 domain binding

Biological Process: blood coagulation; cell adhesion; leukocyte migration