Mouse Shank1 Monoclonal Antibody | anti-SHANK1 antibody

Anti-Shank1 Monoclonal Antibody

Cat. Num. AAA190526

• Gene Names: SHANK1; SSTRIP; SPANK-1; synamon
• Reactivity: Human, mouse, rat
• Applications: Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: Shank1; Monoclonal Antibody; Anti-Shank1 Monoclonal Antibody; anti-SHANK1 antibody

• Host: Mouse
• Reactivity: Human, mouse, rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S22-21
• Specificity: Shank1; This antibody recognizes human, mouse, and rat Shank 1. It does not react with Shank2 or 3.
• Form/Format: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
• Sequence Positions: 469-691

• Applicable Applications for anti-SHANK1 antibody: Immunoblot; Immunohistochemistry; Immunocytochemistry; Immunofluorescence
• Application Notes: Immunoblotting: use at 1-10ug/ml. Bands of ~190-220kDa (alternative splice variants) are detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. Immunofluorescence: use at 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Adult rat brain.
• Antigen: Fusion protein corresponding to aa 469-691 (SH3/PDZ domains) of rat Shank1 (accession no. Q9WV48). This sequence is 96% homologous with human and 100% homologous with mouse Shank1.
• Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.

Testing Data

Related Product Information for anti-SHANK1 antibody

Shank proteins are a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are crucial in receptor tyrosine kinase signaling. Recent studies suggest that disruption of glutamate receptors at the Shank-postsynaptic platform could contribute to the destruction of postsynaptic density which underlies synaptic dysfunction and loss in Alzheimer's disease. Shank1 might be relevant to human autism spectrum disorders due to its differential role in specific cognitive processes along with its importance in synapse structure and function.

NCBI and Uniprot Product Information

• NCBI GI #: 120587025
• NCBI GeneID: 50944
• NCBI Accession #: NP_057232.2
• NCBI GenBank Nucleotide #: NM_016148.2
• UniProt Accession #: Q9Y566; Q9NYW9; A8MXP5; B7WNY6
• Molecular Weight: 224,959 Da
• NCBI Official Full Name: SH3 and multiple ankyrin repeat domains protein 1
• NCBI Official Synonym Full Names: SH3 and multiple ankyrin repeat domains 1
• NCBI Official Symbol: SHANK1
• NCBI Official Synonym Symbols: SSTRIP; SPANK-1; synamon
• NCBI Protein Information: SH3 and multiple ankyrin repeat domains protein 1; OTTHUMP00000174437; OTTHUMP00000174438; SSTR-interacting protein; somatostatin receptor-interacting protein
• UniProt Protein Name: SH3 and multiple ankyrin repeat domains protein 1
• Protein Family: SH3 and multiple ankyrin repeat domains protein
• UniProt Gene Name: SHANK1
• UniProt Entry Name: SHAN1_HUMAN

Uniprot Description

SHANK1: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Belongs to the SHANK family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: postsynaptic membrane; neuron projection; membrane; cytoplasm; dendrite; plasma membrane; dendritic spine; excitatory synapse; ionotropic glutamate receptor complex; intracellular; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein C-terminus binding; somatostatin receptor binding; identical protein binding; ionotropic glutamate receptor binding; protein binding; protein complex binding; GKAP/Homer scaffold activity; SH3 domain binding

Biological Process: habituation; negative regulation of actin filament bundle formation; synapse maturation; determination of affect; olfactory behavior; long-term memory; righting reflex; cytoskeletal anchoring; adult behavior; protein complex assembly; social behavior; neuromuscular process controlling balance; associative learning