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Western Blot (WB) (Western blot analysis of SFPQ on different lysates using anti-SFPQ antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: Daudi Lane 3: Mouse testis Lane 4: Mouse lung)

Rabbit SFPQ Monoclonal Antibody | anti-SFPQ antibody

SFPQ Antibody

Gene Names
SFPQ; PSF; POMP100; PPP1R140
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
ProA affinity purified
Synonyms
SFPQ; Monoclonal Antibody; SFPQ Antibody; 100 kDa DNA pairing protein antibody; 100 kDa DNA-pairing protein antibody; 100 kDa subunit antibody; DNA binding p52/p100 complex 100 kDa subunit antibody; DNA-binding p52/p100 complex antibody; hPOMp100 antibody; Polypyrimidine tract binding protein associated splicing factor antibody; Polypyrimidine tract-binding protein-associated-splicing factor antibody; POMP100 antibody; PPP1R140 antibody; proline- and glutamine-rich antibody; Protein phosphatase 1 regulatory subunit 140 antibody; PSF antibody; PTB associated splicing factor antibody; PTB-associated-splicing factor antibody; Sfpq antibody; SFPQ_HUMAN antibody; Splicing factor antibody; Splicing factor proline and glutamine rich antibody; Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) antibody; Splicing factor proline/glutamine rich antibody; anti-SFPQ antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JU33-99
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-SFPQ antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:10-1:50
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of SFPQ on different lysates using anti-SFPQ antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: Daudi Lane 3: Mouse testis Lane 4: Mouse lung)

Western Blot (WB) (Western blot analysis of SFPQ on different lysates using anti-SFPQ antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: Daudi Lane 3: Mouse testis Lane 4: Mouse lung)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SFPQ antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SFPQ antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SFPQ antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SFPQ antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SFPQ antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SFPQ antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SFPQ antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SFPQ antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SFPQ antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SFPQ antibody. Counter stained with hematoxylin.)
Related Product Information for anti-SFPQ antibody
DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation.
Product Categories/Family for anti-SFPQ antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Calculated MW: 72kDa/76kDa
Observed MW: 115kDa
NCBI Official Full Name
Splicing factor, proline- and glutamine-rich
NCBI Official Synonym Full Names
splicing factor proline/glutamine-rich
NCBI Official Symbol
SFPQ
NCBI Official Synonym Symbols
PSF; POMP100; PPP1R140
NCBI Protein Information
splicing factor, proline- and glutamine-rich; hPOMp100; 100 kDa DNA-pairing protein; PTB-associated splicing factor; PTB-associated-splicing factor; DNA-binding p52/p100 complex, 100 kDa subunit; protein phosphatase 1, regulatory subunit 140; polypyrimidi
UniProt Protein Name
Splicing factor, proline- and glutamine-rich
Protein Family
UniProt Gene Name
SFPQ
UniProt Synonym Gene Names
PSF; hPOMp100; PSF; PTB-associated-splicing factor
UniProt Entry Name
SFPQ_HUMAN

Uniprot Description

PSF: a proline-and glutamine-rich splicing factor. Essential pre-mRNA splicing factor required early in spliceosome formation. Seems to also bind DNA. The active complex is a heterotetramer of two 52 kDa and two 100 kDa subunits. Reported to be a PKC-alpha-binding protein in the cell nucleus. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nucleus; paraspeckles; chromatin

Molecular Function: protein binding; histone deacetylase binding; nucleotide binding

Biological Process: alternative nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; RNA splicing; rhythmic process; negative regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; DNA repair; mRNA processing; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm; DNA recombination

Research Articles on SFPQ

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Product Notes

The SFPQ sfpq (Catalog #AAA9436525) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SFPQ Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SFPQ can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:10-1:50. Researchers should empirically determine the suitability of the SFPQ sfpq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SFPQ, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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