Mouse anti-Human SATB2 Monoclonal Antibody | anti-SATB2 antibody

Mouse monoclonal antibody Anti-Human SATB2

Cat. Num. AAA120052

• Gene Names: SATB2; GLSS
• Reactivity: Human
• Applications: Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot
• Synonyms: SATB2; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human SATB2; SATB family member 2 (SATB2); FLJ21474; FLJ32076; KIAA1034; MGC119474;MGC119477; anti-SATB2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SATBA4B10
• Form/Format: Mouse monoclonal anti-human SATB2 antibody in PBS (3.0 mM KCl, 1.5 mM KH₂PO₄, 140 mM NaCl, 8.0 mM Na₂HPO₄ (pH 7.4) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN₃)
• Sequence Length: 5306

• Applicable Applications for anti-SATB2 antibody: Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: WB: 0.2-2 ug/ml; IP: 100-500 ug/sample; ICC: 2-100 ug/ml
• Immunogen: Recombinant
• Preparation: This antibody was purified using protein A column chromatography from ascites.
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: This antibody solution contains sodium azide (NaN₃) as a preservative. There is a potential hazard that NaN₃ reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
• Preparation and Storage: Store at 2-8°C for up to one year. ; We recommend storing at -20°C for long-term storage.; Avoid repeat freezing and thawing cycles

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-SATB2 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of SATB2 by Western blot.Samples: Whole cell lysate from human HT1080 (H, 25 ug) and mouse NIH3T3 (M, 25 ug) cells. [Lot No. SATBA4B10-2]Predicted molecular weight: 82 kDa)

Immunoprecipitation (IP)

(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-SATB2 mAb. [Lot No. SATBA4B10-1]Predicted molecular weight: 82 kDa)

Immunocytochemistry (ICC)

(Immunostaining analysis in HT1080 cells. HT1080 cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-SATB2 mAb. [Lot No. SATBA4B10-1])

Related Product Information for anti-SATB2 antibody

May play an important role in palate formation. [UniProtKB Function]

NCBI and Uniprot Product Information

• NCBI GI #: 289547592
• NCBI GeneID: 23314
• NCBI Accession #: NP_056080.1
• NCBI GenBank Nucleotide #: NM_015265
• Molecular Weight: 69,137 Da
• NCBI Official Full Name: Homo sapiens SATB homeobox 2 (SATB2), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: SATB homeobox 2
• NCBI Official Symbol: SATB2
• NCBI Official Synonym Symbols: GLSS
• NCBI Protein Information: DNA-binding protein SATB2; SATB family member 2; special AT-rich sequence-binding protein 2
• UniProt Protein Name: DNA-binding protein SATB2
• Protein Family: DNA-binding protein
• UniProt Gene Name: SATB2
• UniProt Entry Name: SATB2_HUMAN

NCBI Description

This gene encodes a DNA binding protein that specifically binds nuclear matrix attachment regions. The encoded protein is involved in transcription regulation and chromatin remodeling. Defects in this gene are associated with isolated cleft palate and mental retardation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Feb 2010]

Uniprot Description

SATB2: Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper- layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11). Defects in SATB2 are a cause of cleft palate isolated (CPI). A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis. A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22). Belongs to the CUT homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: nucleoplasm; transcription factor complex; nuclear matrix; cytoplasm; histone deacetylase complex

Molecular Function: protein binding; sequence-specific DNA binding; chromatin binding

Biological Process: chromatin remodeling; transcription, DNA-dependent; commitment of a neuronal cell to a specific type of neuron in the forebrain; cartilage development; neuron migration; positive regulation of transcription from RNA polymerase II promoter; palate development; negative regulation of transcription from RNA polymerase II promoter; embryonic pattern specification; embryonic skeletal morphogenesis; osteoblast development

Disease: Glass Syndrome