Cystatin E / CST6 Recombinant Protein | CST6 recombinant protein

Recombinant Human Cystatin E / CST6 Protein (His tag)

Cat. Num. AAA2546258

• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Cystatin E / CST6; Recombinant Human Cystatin E / CST6 Protein (His tag); CST6; cystatin 6; cystatin M; cystatin M/E; cysteine proteinase inhibitor; CST6 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM MES, 0.15M NaCl, pH 6.5
• Sequence Length: 149

• Application Notes: The mature form of recombinant human cystatin E/M consists of 132 amino acids and predicts a molecular mass of 15 kDa. It exists as two major forms with an identical N-terminal sequence. As a result of glycosylation, rh cystatin E/M migrates as doublets with the molecular mass of approximately 15 and 21 kDa respectively in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Arg 29
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CST6 recombinant protein

Background: Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17 kDa) and an unglycosylated (14 kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene.

Description: A DNA sequence encoding the human cystatin E/M protein (NP_001314.1) (Met 1-Met 149) was expressed with a C-terminal polyhistidine-tag.

NCBI and Uniprot Product Information

• NCBI GI #: 4503113
• NCBI GeneID: 1474
• NCBI Accession #: NP_001314.1
• NCBI GenBank Nucleotide #: NM_001323.3
• UniProt Accession #: Q15828; Q540N7
• Molecular Weight: 16,511 Da
• NCBI Official Full Name: cystatin-M
• NCBI Official Synonym Full Names: cystatin E/M
• NCBI Official Symbol: CST6
• NCBI Protein Information: cystatin-M
• UniProt Protein Name: Cystatin-M
• Protein Family: Cystatin
• UniProt Gene Name: CST6

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype. [provided by RefSeq, Jul 2008]

Uniprot Description

CST6: Shows moderate inhibition of cathepsin B but is not active against cathepsin C. Belongs to the cystatin family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: extracellular space

Molecular Function: protease binding

Biological Process: anatomical structure morphogenesis