Mouse anti-Human SATB1 Monoclonal Antibody | anti-SATB1 antibody

SATB1 (233) Mouse mAb

Cat. Num. AAA435175

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Synonyms: SATB1; Monoclonal Antibody; SATB1 (233) Mouse mAb; DNA-binding protein SATB1; special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA); Special AT-rich sequence-binding protein 1; anti-SATB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 763

• Applicable Applications for anti-SATB1 antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:5000; IP: 10 tests; FC/FACS: 1:100-1:200
• Source Note: This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human SATB1
• Preparation and Storage: Store at -20 degree C. Stable for one year from the date of shipment.

Western Blot (WB)

Flow Cytometry (FC/FACS)

Related Product Information for anti-SATB1 antibody

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-SATB1 antibody

Cell Marker; Subcellular Marker; Nucleoplasma

NCBI and Uniprot Product Information

• NCBI GI #: 196114999
• NCBI GeneID: 6304
• NCBI Accession #: NP_001124482.1
• NCBI GenBank Nucleotide #: NM_001131010.3
• UniProt Accession #: Q01826; Q59EQ0; B3KXF1; C9JTR6
• Molecular Weight: Predicted MW: 86kDa
• NCBI Official Full Name: DNA-binding protein SATB1 isoform 1
• NCBI Official Synonym Full Names: SATB homeobox 1
• NCBI Official Symbol: SATB1
• NCBI Protein Information: DNA-binding protein SATB1
• UniProt Protein Name: DNA-binding protein SATB1
• Protein Family: DNA-binding protein
• UniProt Gene Name: SATB1

NCBI Description

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. [provided by RefSeq, Apr 2016]

Uniprot Description

Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.