Rabbit SARS-CoV-2 (2019-nCoV) Spike Neutralizing Monoclonal Antibody | anti-SARS-CoV-2 antibody

SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody, Rabbit Mab

Cat. Num. AAA8119554

• Reactivity: SARS-CoV-2
• Applications: ELISA, Flow Cytometry, Functional Assay, Neutralization Assay
• Purity: Protein A
• Synonyms: SARS-CoV-2 (2019-nCoV) Spike Neutralizing; Monoclonal Antibody; SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody; Rabbit Mab; anti-SARS-CoV-2 antibody

• Host: Rabbit
• Reactivity: SARS-CoV-2
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 118
• Specificity: SARS-CoV-2 (2019-nCoV) Spike RBD Protein
• Purity/Purification: Protein A
• Form/Format: Liquid. 0.22um filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80

• Applicable Applications for anti-SARS-CoV-2 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Neutralization
• Application Notes: ELISA: 0.5-1ug/mL; FC/FACS/FCM: 10-40ug/ml; Antibody's applications have not been validated with corresponding viruses.
• Immunogen: Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein
• Endotoxin: <3EU/mg
• Conjugation: Unconjugated
• Preparation: This product is a recombinant monoclonal antibody expressed from HEK293 cells.
• Cross-reactivity: Has cross-reactivity in ELISA with; SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD Protein ; No cross-reactivity in ELISA with; SARS-CoV (Isolate Tor2) Spike S1+S2 ECD Protein ; MERS-CoV Spike S1+S2 ECD Protein ; HCoV-NL63 Spike S1+S2 ECD Protein ; HCoV-229E Spike S1+S2 ECD Protein ; HCoV-HKU1 (isolate N5) Spike S1+S2 ECD Protein ; HCoV-OC43 Spike S1+S2 ECD Protein
• Notes: Microneutralizaiton (MN): The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on 293T-ACE2 cells infected with SARS-CoV-2 (2019-nCoV) Spike Pseudovirus under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody. Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC50 is typically 0.305ug/mL.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

ELISA (EIA)

(Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody was detected by SARS-CoV-2 (2019-nCoV) Inhibitor Screening ELISA Kit. The IC50 is typically 0.5-5 nM.)

Testing Data

(The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on 293T-ACE2 cells infected with SARS-CoV-2 (2019-nCoV) Spike Pseudovirus under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody. Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC50 is typically 0.185 ug/mL.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of SARS-COV-2 Spike overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Spike Neutralizing Rabbit MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-SARS-CoV-2 antibody

Background: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

References

Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.