SARS-CoV/SARS-CoV-2 Spike Monoclonal Antibody | anti-SARS-CoV/SARS-CoV-2 antibody

SARS-CoV/SARS-CoV-2 Spike antibody,Chimeric MAb

Cat. Num. AAA8119477

• Reactivity: SARS
• Applications: ELISA
• Purity: Protein A
• Synonyms: SARS-CoV/SARS-CoV-2 Spike; Monoclonal Antibody; SARS-CoV/SARS-CoV-2 Spike antibody; Chimeric MAb; Anti-coronavirus s1 Antibody; Anti-coronavirus s2 Antibody; Anti-coronavirus spike Antibody; Anti-cov spike Antibody; Anti-ncov RBD Antibody; Anti-ncov s1 Antibody; Anti-ncov s2 Antibody; Anti-ncov spike Antibody; Anti-novel coronavirus RBD Antibody; Anti-novel coronavirus s1 Antibody; Anti-novel coronavirus s2 Antibody; Anti-novel coronavirus spike Antibody; Anti-RBD Antibody; Anti-S1 Antibody; Anti-s2 Antibody; Anti-Spike RBD Antibody; anti-SARS-CoV/SARS-CoV-2 antibody

• Host: Chimeric Mouse/Human
• Reactivity: SARS
• Clonality: Monoclonal
• Isotype: Mouse (variable region)/Human (kappa/IgG1 constant) chimeric antibody
• Clone Number: D004
• Specificity: SARS-CoV Spike RBD Protein
• Purity/Purification: Protein A
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-SARS-CoV/SARS-CoV-2 antibody: ELISA (EIA)
• Application Notes: ELISA: 1:5000-1:10,000; Antibody's applications haven't been validated with corresponding virus positive samples.
• Immunogen: Recombinant SARS-CoV Spike RBD Protein
• Conjugation: Unconjugated
• Preparation: It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant SARS-CoV Spike RBD Protein. The antibody was produced using recombinant antibody technology.
• Cross-reactivity: Has cross-reactivity in ELISA with; SARS-CoV Spike S1 Protein ; SARS-CoV-2 (2019-nCoV) Spike S1 Protein ; SARS-CoV-2 (2019-nCoV) Spike RBD Protein ; No cross-reactivity in ELISA with; MERS-CoV Spike S1 Protein ; HCoV-HKU1 (isolate N1) Spike S1 Protein ; HCoV-HKU1 (isolate N5) Spike S1 Protein ; HCoV-NL63 Spike S1 Protein ; HCoV-229E Spike S1 Protein ; HCoV-OC43 Spike S1+S2 ECD Protein
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Immunofluorescence (IF)

(Immunofluorescence staining of SARS-CoV-2 (2019-nCoV) Spike in ACE2-overexpressed 293T cells, infected (left) or noninfected (right) by 2019-nCOV-Spike pseudovirus (PSV). Cells were blocked with 10% serum, and incubated with SARS-CoV Spike monoclonal antibody (dilution ratio 1:60) at 37 degree C for 1 hour. Then cells were stained with the FITC-conjugated Goat Anti-human IgG secondary antibody (green).)

Related Product Information for anti-SARS-CoV/SARS-CoV-2 antibody

Background: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

References

Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.