Western Blot (WB)
(Western blot analysis of extracts from Human fallopian tube, using DNAH5 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit DNAH5 Polyclonal Antibody | anti-DNAH5 antibody
DNAH5 Antibody
Gene Names
DNAH5; HL1; PCD; CILD3; KTGNR; DNAHC5
Reactivity
Human, Mouse, RatPredicted Reactivity: Rabbit(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
DNAH5; Polyclonal Antibody; DNAH5 Antibody; axonemal; Axonemal beta dynein heavy chain 5; Ciliary dynein heavy chain 5; DNAH 5; Dnah5; DNAHC5; DYH5_HUMAN; Dynein heavy chain 5; HL1; KIAA1603; anti-DNAH5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Rabbit(88%)
Predicted Reactivity: Rabbit(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
DNAH5 Antibody detects endogenous levels of total DNAH5.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
4624
Applicable Applications for anti-DNAH5 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human DNAH5, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Human fallopian tube, using DNAH5 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Human fallopian tube, using DNAH5 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-DNAH5 antibody
Function: Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required for structural and functional integrity of the cilia of ependymal cells lining the brain ventricles.
Subcellular Location: Cytoplasm>Cytoskeleton>Cilium axoneme.
Subunit Structure: Interacts with DNAL1 (PubMed:21496787). Consists of at least two heavy chains and a number of intermediate and light chains.
Similarity: Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.Belongs to the dynein heavy chain family.
Subcellular Location: Cytoplasm>Cytoskeleton>Cilium axoneme.
Subunit Structure: Interacts with DNAL1 (PubMed:21496787). Consists of at least two heavy chains and a number of intermediate and light chains.
Similarity: Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.Belongs to the dynein heavy chain family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)529kD.
Predicted Molecular Weight: (Calculated)529kDa.
Predicted Molecular Weight: (Calculated)529kDa.
NCBI Official Full Name
dynein heavy chain 5, axonemal
NCBI Official Synonym Full Names
dynein axonemal heavy chain 5
NCBI Official Symbol
DNAH5
NCBI Official Synonym Symbols
HL1; PCD; CILD3; KTGNR; DNAHC5
NCBI Protein Information
dynein heavy chain 5, axonemal
UniProt Protein Name
Dynein heavy chain 5, axonemal
Protein Family
UniProt Gene Name
DNAH5
UniProt Synonym Gene Names
DNAHC5; HL1; KIAA1603
UniProt Entry Name
DYH5_HUMAN
NCBI Description
This gene encodes a dynein protein, which is part of a microtubule-associated motor protein complex consisting of heavy, light, and intermediate chains. This protein is an axonemal heavy chain dynein. It functions as a force-generating protein with ATPase activity, whereby the release of ADP is thought to produce the force-producing power stroke. Mutations in this gene cause primary ciliary dyskinesia type 3, as well as Kartagener syndrome, which are both diseases due to ciliary defects. [provided by RefSeq, Oct 2009]
Research Articles on DNAH5
Similar Products
Product Notes
The DNAH5 dnah5 (Catalog #AAA9612343) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAH5 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Rabbit(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's DNAH5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the DNAH5 dnah5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAH5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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