anti-Human SAFB1 Monoclonal Antibody | anti-SAFB1 antibody

Anti-SAFB1 Mouse mAb

Cat. Num. AAA475139

• Gene Names: SAFB; HAP; HET; SAF-B; SAFB1; SAB-B1; SAF-B1
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity Purified
• Synonyms: SAFB1; Monoclonal Antibody; Anti-SAFB1 Mouse mAb; DKFZp779C1727; glutathione S transferase fusion protein; HAP; HET; HSP27 ERE TATA binding protein; HSP27 ERE-TATA-binding protein; HSP27 estrogen response element-TATA box-binding protein; SAF B; SAF-B1; SAFB 1; SAFB; SAFB1_HUMAN; scaffold attachment factor B1; anti-SAFB1 antibody

• Host: Mouse; Source: Human
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2E8-E2-G6
• Purity/Purification: Affinity Purified
• Form/Format: Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 917

• Applicable Applications for anti-SAFB1 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000; ICC: 1:200
• Immunogen: Purified recombinant human SAFB1 protein fragments expressed in E Coli.
• Sensitivity: This antibody detects endogenous levels of SAFB1 and does not cross-react with related prote
• Sequence Similarities: Contains 1 RRM (RNA recognition motif) domain.Contains 1 SAP domain.
• Subunit Structure: Monomer. Can form homodimers. Interacts with KHDRBS3, POLR2A, SAFB2 or SFRS1, SFRS9 and TRA2B/SFRS10 By similarity. Interacts with isoform 1 and isoform 2 of SRPK1 and inhibits its activity. Interacts with RBMX By similarity.
• Subcellular Location: Nucleus

Western Blot (WB)

(Western blot detection of SAFB1 in HelaNE, A431, MDA-MB-468, Breast cancer, ZR75-1 and MCF7 cell lysates using SAFB1 mouse mAb (1:4000 diluted).Predicted band size: 130kDa.Observed band size: 130kDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using SAFB1 mouse mAb (dilution 1:200).)

Related Product Information for anti-SAFB1 antibody

Entrez Summary: This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.
UniPort Summary Function: Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing By similarity.

Product Categories/Family for anti-SAFB1 antibody

Epigenetics and Nuclear Signaling; Chromosome Structure; Scaffold Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 321267469
• NCBI GeneID: 6294
• NCBI Accession #: NP_001188267.1
• NCBI GenBank Nucleotide #: NM_002967.2
• UniProt Accession #: Q15424; O60406; Q59HH8; A0AV56; B7Z5B6; B7ZLP6; F5H0H3
• Molecular Weight: 130
• NCBI Official Full Name: scaffold attachment factor B1 isoform 1
• NCBI Official Synonym Full Names: scaffold attachment factor B
• NCBI Official Symbol: SAFB
• NCBI Official Synonym Symbols: HAP; HET; SAF-B; SAFB1; SAB-B1; SAF-B1
• NCBI Protein Information: scaffold attachment factor B1
• UniProt Protein Name: Scaffold attachment factor B1
• UniProt Gene Name: SAFB
• UniProt Synonym Gene Names: HAP; HET; SAFB1; SAF-B; SAF-B1; HSP27 ERE-TATA-binding protein

NCBI Description

This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]

Uniprot Description

SAFB1: Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter. Can inhibit cell proliferation.

Protein type: DNA-binding; Nuclear receptor co-regulator; RNA-binding

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: double-stranded DNA binding; protein binding; sequence-specific DNA binding

Biological Process: establishment and/or maintenance of chromatin architecture; estrogen receptor signaling pathway; regulation of mRNA processing; regulation of transcription from RNA polymerase II promoter