Rabbit anti-Human S100P Monoclonal Antibody | anti-S100P antibody

Recombinant Anti-S100P Antibody, Rabbit Monoclonal

Cat. Num. AAA8106561

• Gene Names: S100P; MIG9
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: S100P; Monoclonal Antibody; Recombinant Anti-S100P Antibody; Rabbit Monoclonal; S100P Antibody; Rabbit MAb; S100 calcium binding protein P; Anti-MIG9 Antibody; anti-S100P antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 116
• Specificity: Human S100P.; No cross-reactivity in ELISA with Human S100A1, Mouse S100A9, Human S100A11, Human S100A9, Human S100A10
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-S100P antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:100-1:500; ICC/IF: 1:20-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human S100P protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human S100P (rh S100P).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human S100P in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human S100P in human placenta with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). The image showing nucleus staining of trophoblastic cells.)

Immunofluorescence (IF)

(Immunofluorescence staining of S100P in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Y S100P monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus and Cytoplasm.)

Related Product Information for anti-S100P antibody

Protein S100-P, also known as Protein S100-E, S100 calcium-binding protein P, S100P and S100E, is a nucleus and cytoplasm protein that belongs to the S-100 family. S100P/S100E contains twoEF-hand domains. S100P protein regulates calcium signal transduction and mediates cytoskeletal interaction, protein phosphorylation and transcriptional control. S100P/S100E overexpression can upregulate androgen receptor expression and thereby promote prostate cancer progression by increasing cell growth. S100P/S100E may directly confer resistance to chemotherapy. S100P/S100E induction may be considered an important step in the initial stage of lung adenocarcinomas, whereas its downregulation in advanced stages seems to be important for tumour progression in which DNA methylation and/or feedback transcription processes play a critical role. S100P/S100E plays a major role in the aggressiveness of pancreatic cancer that is likely mediated by its ability to activate RAGE. Interference with S100P/S100E may provide a novel approach for treatment of pancreatic cancer. S100P/S100E could be considered a potential drug target or a chemosensitization target, and could also serve as a biomarker for aggressive, hormone-refractory and metastatic prostate cancer.

Product Categories/Family for anti-S100P antibody

Calcium-binding Proteins and Related Molecules

References

Arumugam, T. et al., 2005, Clin Cancer Res. 11 (15): 5356-64. Basu, G.D. et al., 2008, Int J Cancer. 123 (2): 330-9. Rehbein, G. et al., 2008, Int J Mol Med. 22 (1): 69-77. Heil, A. et al., 2011, J Biol Chem. 286 (9): 7227-38.

NCBI and Uniprot Product Information

• NCBI GI #: 119602790
• NCBI GeneID: 6286
• UniProt Accession #: P25815; Q5J7W2
• Molecular Weight: 10,400 Da
• NCBI Official Full Name: S100 calcium binding protein P
• NCBI Official Synonym Full Names: S100 calcium binding protein P
• NCBI Official Symbol: S100P
• NCBI Official Synonym Symbols: MIG9
• NCBI Protein Information: protein S100-P; protein S100-E; S100 calcium-binding protein P; migration-inducing gene 9 protein
• UniProt Protein Name: Protein S100-P
• Protein Family: S100P-binding protein
• UniProt Gene Name: S100P
• UniProt Synonym Gene Names: S100E; MIG9
• UniProt Entry Name: S100P_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

S100P: Homodimer. Interacts with S100PBP and S100Z. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: microvillus membrane; cytoplasm; nucleus

Molecular Function: protein binding; RAGE receptor binding; magnesium ion binding; calcium ion binding; calcium-dependent protein binding

Biological Process: response to organic substance; endothelial cell migration