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Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (SPM354).)

Mouse S100B Monoclonal Antibody | anti-S100B antibody

S100B (Astrocyte and Melanoma Marker)

Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat, Cow. Others not known.
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
S100B; Monoclonal Antibody; S100B (Astrocyte and Melanoma Marker); NEF; Protein S100-B; S-100 protein beta chain; S100 calcium binding protein beta (neural); S100 calcium-binding protein B; S100 protein beta chain; S100beta; anti-S100B antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat, Cow. Others not known.
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
SPM354
Specificity
S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
92
Applicable Applications for anti-S100B antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes
FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml
WB: 0.5-1ug/ml
IHC-F: 0.5-1ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
Purified bovine brain S100 protein
Cellular Localization
Cytoplasmic
Hu-chromosome Location
21q22.3
Positive Control
Brain, Melanoma
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (SPM354).)

Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (SPM354).)
References
Joshi MG et. al. Modern Pathology, 1996, 9(1):57-62. McLaren KM et. al. Human Pathology, 1996, 27(7):633-6.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10-12kDa
NCBI Official Full Name
protein S100-B
NCBI Official Synonym Full Names
S100 calcium binding protein B
NCBI Official Symbol
S100B
NCBI Official Synonym Symbols
NEF; S100; S100-B; S100beta
NCBI Protein Information
protein S100-B
UniProt Protein Name
Protein S100-B
Protein Family
UniProt Gene Name
S100B

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

Uniprot Description

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.

Research Articles on S100B

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Product Notes

The S100B s100b (Catalog #AAA4380218) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The S100B (Astrocyte and Melanoma Marker) reacts with Human, Mouse, Rat, Cow. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's S100B can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin. FC/FACS: 0.5-1ug/million cells IF: 1-2ug/ml WB: 0.5-1ug/ml IHC-F: 0.5-1ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the S100B s100b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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