Mouse Ryanodine Receptor 1,2 Monoclonal Antibody | anti-RYR1 antibody

Ryanodine Receptor 1,2 (RyR)

Cat. Num. AAA600620

• Gene Names: RYR1; CCO; MHS; RYR; MHS1; RYDR; SKRR; RYR-1
• Reactivity: Bovine, Canine, Chicken, Frog, Human, Mouse, Rat, Sheep
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Ascites; Neat ascites
• Synonyms: Ryanodine Receptor 1; 2; Monoclonal Antibody; 2 (RyR); Anti -Ryanodine Receptor 1; anti-RYR1 antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Chicken, Frog, Human, Mouse, Rat, Sheep
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8.BI.500
• Specificity: Recognizes 565kD RyR-1 and RyR-2 in bovine, canine, human, mouse, rat and sheep. Crossreacts with chicken RyRa, RyRb and cardiac isoforms and frog RyRa and RyRb isoforms.
• Purity/Purification: Ascites; Neat ascites
• Form/Format: Supplied as a liquid with 0.05% sodium azide.

• Applicable Applications for anti-RYR1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in WesternBlot, Immunohistochemistry, Immunoprecipitation and Immunofluorescence.; Dilution: Western Blot: 1:5000; Immunohistochemistry: 1:1000
• Immunogen: Partially purified chicken pectoral muscle ryanodine receptor.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-RYR1 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 7656700
• NCBI GeneID: 6261
• UniProt Accession #: O75591; P21817; Q59FN5; Q8WYM7; Q8WYM8
• Molecular Weight: 13,237 Da
• NCBI Official Full Name: ryanodine receptor 1
• NCBI Official Synonym Full Names: ryanodine receptor 1 (skeletal)
• NCBI Official Symbol: RYR1
• NCBI Official Synonym Symbols: CCO; MHS; RYR; MHS1; RYDR; SKRR; RYR-1
• NCBI Protein Information: ryanodine receptor 1; ryanodine receptor type1; central core disease of muscle; type 1-like ryanodine receptor; skeletal muscle ryanodine receptor; skeletal muscle calcium release channel; sarcoplasmic reticulum calcium release channel
• UniProt Protein Name: Type 1-like ryanodine receptor
• Protein Family: Ryanodine receptor
• UniProt Entry Name: O75591_HUMAN

NCBI Description

This gene encodes a ryanodine receptor found in skeletal muscle. The encoded protein functions as a calcium release channel in the sarcoplasmic reticulum but also serves to connect the sarcoplasmic reticulum and transverse tubule. Mutations in this gene are associated with malignant hyperthermia susceptibility, central core disease, and minicore myopathy with external ophthalmoplegia. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq]

Uniprot Description

RYR1: Calcium channel that controls communication between transverse-tubules and sarcoplasmic reticulum. Contraction of skeletal muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Can mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal development of muscle fibers, skeletal muscle, heart morphogenesis, and skin development and ossification during embryogenesis. Defects in RYR1 are the cause of malignant hyperthermia susceptibility type 1 (MHS1) and central core disease of muscle (CCD). CCD is an autosomal dominant congenital myopathy, but a severe autosomal recessive form also exists. Defects in RYR1 are the cause of multiminicore disease with external ophthalmoplegia (MMDO), congenital myopathy with fiber-type disproportion (CFTD). Defects in RYR1 may be a cause of Samaritan myopathy, a congenital myopathy with benign course. Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Channel, calcium; Transporter; Transporter, ion channel; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: I band; sarcoplasmic reticulum membrane; smooth endoplasmic reticulum; sarcoplasmic reticulum; integral to plasma membrane; cytoplasm; T-tubule; junctional membrane complex; plasma membrane; cell cortex

Molecular Function: calmodulin binding; voltage-gated calcium channel activity; protein binding; protease binding; calcium channel activity; calcium-release channel activity; ryanodine-sensitive calcium-release channel activity

Biological Process: skin development; cytosolic calcium ion homeostasis; muscle contraction; calcium ion transport; release of sequestered calcium ion into cytosol; response to hypoxia; skeletal muscle fiber development; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; transmembrane transport; response to caffeine

Disease: Minicore Myopathy With External Ophthalmoplegia; Malignant Hyperthermia, Susceptibility To, 1; Central Core Disease Of Muscle; Myopathy, Congenital, With Fiber-type Disproportion