Mouse anti-Human RTKN / Rhotekin Monoclonal Antibody | anti-RTKN antibody

Mouse Monoclonal [clone 2E5] (IgG1,k) to Human RTKN / Rhotekin

Cat. Num. AAA245704

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: RTKN / Rhotekin; Monoclonal Antibody; Mouse Monoclonal [clone 2E5] (IgG1; k) to Human RTKN / Rhotekin; Anti-RTKN / Rhotekin Antibody (clone 2E5) IHC-plus; RTKN; Rhotekin; RTKN1; Human RTKN; anti-RTKN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2E5
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 563

• Applicable Applications for anti-RTKN antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (10 - 20 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control A-431. Sandwich ELISA (Recombinant protein).
• Target Species: Human
• Immunogen Description: RTKN (NP_149035, 451 a.a. ~ 550 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: RTKN / Rhotekin antibody was raised against rTKN (NP_149035, 451 a.a. ~ 550 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-RTKN antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(RTKN monoclonal antibody clone 2E5 Western blot of RTKN expression in A-431.)

ELISA (EIA)

(Detection limit for recombinant GST tagged RTKN is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 62739179
• NCBI GeneID: 6242
• NCBI Accession #: NP_001015055.1
• NCBI GenBank Nucleotide #: NM_001015055.1
• UniProt Accession #: Q9BST9; Q8WVN1; Q96PT6; Q9HB05; H7BXD4
• Molecular Weight: 56,666 Da
• NCBI Official Full Name: rhotekin isoform a
• NCBI Official Synonym Full Names: rhotekin
• NCBI Official Symbol: RTKN
• NCBI Protein Information: rhotekin; RTKN
• UniProt Protein Name: Rhotekin
• Protein Family: Rhotekin
• UniProt Gene Name: RTKN
• UniProt Entry Name: RTKN_HUMAN

NCBI Description

This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

RTKN: Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein regulator, misc.

Chromosomal Location of Human Ortholog: 2p13.1

Molecular Function: protein binding; GTP binding; GTP-Rho binding; GTPase inhibitor activity

Biological Process: apoptosis; negative regulation of catalytic activity; signal transduction; Rho protein signal transduction