Rabbit anti-Human CDKN2B Polyclonal Antibody | anti-CDKN2B antibody

CDKN2B, CT (CDKN2B, MTS2, Cyclin-dependent kinase 4 inhibitor B, Multiple tumor suppressor 2, p14-INK4b, p15-INK4b) (Biotin)

Cat. Num. AAA6284701

• Gene Names: CDKN2B; P15; MTS2; TP15; CDK4I; INK4B; p15INK4b
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CDKN2B; Polyclonal Antibody; CT (CDKN2B; MTS2; Cyclin-dependent kinase 4 inhibitor B; Multiple tumor suppressor 2; p14-INK4b; p15-INK4b) (Biotin); p15-INK4b; anti-CDKN2B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CDKN2B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CDKN2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-131 amino acids from the C-terminal region of human CDKN2B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CDKN2B Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CDKN2B antibody detected the CDKN2B protein (arrow).)

Related Product Information for anti-CDKN2B antibody

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.

Product Categories/Family for anti-CDKN2B antibody

Antibodies; Protein Kinase Inhibitors

References

Camacho, C.V., et al. Carcinogenesis 31(10):1889-1896(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Pechlivanis, S., et al. Arterioscler. Thromb. Vasc. Biol. 30(9):1867-1872(2010). Heni, M., et al. Diabetes (2010). In press: Roder, C., et al. Childs Nerv Syst (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 17981694
• NCBI GeneID: 1030
• NCBI Accession #: NP_004927.2; NP_511042.1
• NCBI GenBank Nucleotide #: NM_004936.3
• UniProt Accession #: P42772; O15125; Q6FI09; AAB32712
• Molecular Weight: 14,722 Da
• NCBI Official Full Name: cyclin-dependent kinase 4 inhibitor B isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
• NCBI Official Symbol: CDKN2B
• NCBI Official Synonym Symbols: P15; MTS2; TP15; CDK4I; INK4B; p15INK4b
• NCBI Protein Information: cyclin-dependent kinase 4 inhibitor B; MTS-2; p14-INK4b; p14_INK4B; p15-INK4b; p15_INK4B; CDK4B inhibitor; p14_CDK inhibitor; p15 CDK inhibitor; CDK inhibitory protein; multiple tumor suppressor 2; cyclin-dependent kinases 4 and 6 binding protein
• UniProt Protein Name: Cyclin-dependent kinase 4 inhibitor B
• Protein Family: Cyclin-dependent kinase 4 inhibitor
• UniProt Gene Name: CDKN2B
• UniProt Synonym Gene Names: MTS2; MTS-2; p15INK4B
• UniProt Entry Name: CDN2B_HUMAN

NCBI Description

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

p15-INK4B: a potent inhibitor that nteracts strongly with CDK4 and CDK6. Potential effector of TGF-beta induced cell cycle arrest.

Protein type: Cell cycle regulation; Inhibitor; Tumor suppressor; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; protein kinase binding

Biological Process: spleen development; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; megakaryocyte differentiation; mitotic cell cycle checkpoint; positive regulation of transforming growth factor beta receptor signaling pathway; cellular response to nutrient; liver development; response to organic cyclic substance; negative regulation of cell proliferation; transforming growth factor beta receptor signaling pathway; response to cytokine stimulus; cellular response to extracellular stimulus; positive regulation of epithelial cell differentiation; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; gene expression; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; cell cycle arrest; G2/M transition of mitotic cell cycle; negative regulation of epithelial cell proliferation; aging