Rat anti-Mouse RSPO-3 Monoclonal Antibody | anti-RSPO1 antibody

RSPO-3 (R-Spondin 3, Cristin-1)

Cat. Num. AAA603109

• Gene Names: RSPO1; RSPO; CRISTIN3; RP11-566C13.1
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: RSPO-3; Monoclonal Antibody; RSPO-3 (R-Spondin 3; Cristin-1); Anti -RSPO-3 (R-Spondin 3; anti-RSPO1 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 7H222
• Specificity: Recognizes mouse RSPO-3. No crossreactivity with recombinant mouse RSPO-1, RSPO-4, recombinant human RSPO-2 or RSPO-3.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS, pH 7.4.

• Applicable Applications for anti-RSPO1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Direct ELISA and Western Blot.; Dilution: Western Blot: 1ug/ml; Optimal dilutions to be determined by researcher.
• Immunogen: Recombinant corresponding to aa21-209 of mouse RSPO-3 expressed in E. coli (Accession #BAC36296).
• Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant mouse RSPO-3.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-RSPO1 antibody

RSPO-3 (R-spondin 3; also Cristin-1), is a 256aa secreted R-Spondin family member which binds LRP-6 and Fzd8 and regulates b-catenin signaling. R-Spondins contain two adjacent cysteine-rich furin-like domains at their amino-termini, followed by a thrombospondin (TSP-1) motif and a variable length basic C-terminal region. Two RSPO-3 splice isoforms that differ in their C-termini have been reported. Mouse RSPO-3 shares 93% and 97% aa identity with human and rat RSPO-3, respectively, over the sequence used as an immunogen.

Product Categories/Family for anti-RSPO1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 339276103
• NCBI GeneID: 284654
• NCBI Accession #: NP_001229839.1
• NCBI GenBank Nucleotide #: NM_001242910.1
• UniProt Accession #: Q2MKA7; Q0H8S6; Q14C72; Q5T0F2; Q8N7L5; A2A420
• Molecular Weight: 28,959 Da
• NCBI Official Full Name: R-spondin-1 isoform 3
• NCBI Official Synonym Full Names: R-spondin 1
• NCBI Official Symbol: RSPO1
• NCBI Official Synonym Symbols: RSPO; CRISTIN3; RP11-566C13.1
• NCBI Protein Information: R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
• UniProt Protein Name: R-spondin-1
• Protein Family: R-spondin
• UniProt Gene Name: RSPO1
• UniProt Synonym Gene Names: hRspo1
• UniProt Entry Name: RSPO1_HUMAN

NCBI Description

This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. [provided by RefSeq, Jul 2008]

Uniprot Description

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: extracellular space; extracellular region; nucleus

Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding

Biological Process: regulation of gene expression; regulation of receptor internalization; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation; male meiosis; positive regulation of Wnt receptor signaling pathway

Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal